Kukho amagorha amaninzi angaculwanga asijikelezileyo, abonakala eqhelekile, kodwa eneneni athi cwaka anegalelo elikhulu kuthi.Iproteinase K "ligorha elingachazwanga" kwishishini lokuxilonga imolekyuli, nangona ithelekiswa "nenkulu kwaye inamandla" kwishishini, iproteinase K iphantsi kakhulu kangangokuba kudala sikuhoya ukubaluleka kwayo.Ngokuqhambuka kwesibetho esitsha sesithsaba, imfuno yeproteinase K iye yanda, kwaye unikezelo ekhaya nakwamanye amazwe lusemva kakhulu kusetyenziso, kwaye wonke umntu ngequbuliso waqonda ukuba iproteinase K ibaluleke kakhulu.
Yintoni ukusetyenziswa kweproteinase K?
I-Proteinase K i-serine protease kunye nomsebenzi we-proteolytic enzyme kwaye inokugcina umsebenzi kwiindawo ezininzi ezingqongileyo (pH (4-12.5), i-buffer ephezulu yetyuwa, ukushisa okuphezulu kwe-70 ° C, njl.).Ukongezelela, umsebenzi we-proteinase K awuthintelwa yi-SDS, urea, i-EDTA, i-guanidine hydrochloride, i-guanidine isothiocyanate, njl. ), ukutya (inyama yethenda), isikhumba (ienwele ezithambileyo), ukwenziwa kwewayini (ukucaciswa kotywala), ukulungiswa kwe-amino acid (iintsiba ezonakaliswe), i-nucleic acid extraction, in situ hybridization, njl., proteinase K Kukho izicelo.Esona sicelo sisetyenziswa ngokuqhelekileyo yi-nucleic acid extraction.
Iproteinase K inokwenza i-enzymolyze zonke iintlobo zeeprotheni kwisampulu, kubandakanywa nezo histones ezibotshelelwe ngokuqinileyo kwi-nucleic acids, ukwenzela ukuba i-nucleic acids ikhutshwe kwisampuli kwaye ikhutshwe kwisicatshulwa, iququzelele inyathelo elilandelayo lokutsalwa kunye nokuhlanjululwa.Ekubhaqweni kwe-viral nucleic acid, iproteinase K yenye yezinto ezibalulekileyo kwisisombululo sesampulu yentsholongwane.I-Proteinase K inokuqhekeka kwaye ingasebenzi iprotheni yengubo yentsholongwane, ekhuselekileyo ngexesha lokuthutha kunye nenqanaba lokufumanisa;Ukongeza, i-proteinase K inokuthoba i-RNase ikhusela ukuthotywa kwe-RNA yentsholongwane kwaye iququzelele ukubonwa kwe-nucleic acid.
Udumo lobusuku beproteinase K
Nokuba kuphando lwezenzululwazi okanye kwinkalo ye-IVD, ukutsalwa kwe-nucleic acid lolona vavanyo lusisiseko, ngoko ke iproteinase K ibisoloko iyinto ebaluleke kakhulu.Nangona kunjalo, kwixesha elidlulileyo, iproteinase K yayingaziwa kakhulu kunendima yayo.Inxalenye enkulu yoku yayingenxa yokuba unikezelo kunye nobudlelwane bemfuno yeproteinase K babuzinzile kakhulu.Bambalwa abantu abanokucinga ukuba unikezelo lweproteinase K lunokuba yingxaki.
Ngokuqhambuka kobhubhani omtsha wesithsaba, imfuno yovavanyo lwe-nucleic acid iye yanda.Ukusukela ngasekupheleni kukaJuni ka-2020, iTshayina igqibe malunga nezigidi ezingama-90 zovavanyo lwesithsaba, kwaye eli nani loyikeka ngakumbi kwihlabathi.Kwiimvavanyo ze-nucleic acid extraction, ukuxinwa okusebenzayo kweproteinase K malunga ne-50-200 μg / mL.Ngokuqhelekileyo, kuthatha malunga ne-100 μg yeproteinase K ukukhupha isampuli ye-nucleic acid.Ekusetyenzisweni kwangempela, ukwenzela ukwandisa ukusebenza kakuhle kwe-nucleic acid extraction, ngokuqhelekileyo iProteinase K iya kusetyenziswa kwixabiso elongezelelweyo.Ukufunyanwa kwe-Nucleic acid ye-coronavirus entsha kuzise isixa esikhulu semfuno yeproteinase K.Ubonelelo lokuqala kunye nemfuno elinganayo yeproteinase K yaphuka ngokukhawuleza, kwaye iproteinase K yaba yinto ebalulekileyo yokuthintela ubhubhane ngobusuku nje obunye.
Ubunzima bokuvelisa iproteinase K
Nangona ngokuphuhliswa kobhubhane, ixabiso elibalulekileyo leproteinase K liye laxatyiswa ngabantu, kuyadanisa ukuba ngenxa yokugqithiswa kwesitshixo seproteinase K, iinkampani zasekhaya ezimbalwa ziye zabandakanyeka ekuveliseni iproteinase K. Xa abantu bafuna ukuseka imveliso yeproteinase K Ngexesha lenkqubo yemveliso, kwafunyaniswa ukuba iproteinase K yiprotheyini ekhethekileyo kakhulu.Kungumceli mngeni kakhulu ukwandisa amandla okuvelisa iproteinase K ngexesha elifutshane.
Imveliso enkulu yeproteinase K ijongene nobunzima obulandelayo
1. Ukuchazwa okuphantsi
Iproteinase K ayinakuthoba ngokuthe ngqo uninzi lweeprotheyini kwaye ibangele ubutyhefu obumandla kwintetho yeseli ye-host.Ke ngoko, inqanaba lokubonisa leproteinase K lisezantsi kakhulu.Ukuhlolwa kweendlela zokuthetha kunye neentlobo ezichaza kakhulu iproteinase K ifuna umjikelo omde.
2. Iintsalela zebala kunye ne-nucleic acids
Ukuvubela okukhulu kwazisa inani elikhulu le-pigment kunye neentsalela ze-nucleic acid.Kunzima ukususa obu kungcola ngenkqubo yokucoca elula, kwaye ukuhlanjululwa okuyinkimbinkimbi kwandisa iindleko kunye nokunciphisa izinga lokubuyisela.
3. Ukungazinzi
Iproteinase K ayizinzile ngokwaneleyo, iyakwazi ukwenza i-enzymolyze ngokwayo, kwaye kunzima ukuyigcina ngokuzinzileyo kwi-37 ° C ixesha elide ngaphandle kwe-agent ekhuselayo.
4. Kulula ukugalela
Xa ulungiselela umgubo womkhenkce omisiweyo weproteinase K, ukuze kuqinisekiswe ukuba umxholo oqinileyo weproteinase K kumgubo owomileyo umkhulu, kuyimfuneko ukongeza i-agent ekhuselayo eyomileyo kwiqondo eliphezulu, kodwa xa I-concentration ye-proteinase K ifikelela kwi-20mg / mL nangaphezulu, kulula Ukuhlanganiswa kwenza imvula, ezisa ubunzima obukhulu ekumiseni umkhenkce we-proteinase K kunye nomxholo ophezulu oqinileyo.
5. Utyalo-mali olukhulu
Iproteinase K inomsebenzi owomeleleyo weprotease kwaye inokugalela ezinye iiproteases kwilabhoratri.Ke ngoko, iproteinase K idinga iindawo ezikhethekileyo zemveliso, izixhobo, kunye nabasebenzi bophando kunye nophuhliso kunye nemveliso.
Isisombululo se-XD BIOCHEM's proteinase K
I-XD BIOCHEM ine-protein ekhulileyo yokubonakaliswa kunye neqonga lokuhlanjululwa, kwaye inamava atyebileyo ekuboniseni nasekuhlanjululeni iiprotheni eziphinda ziphinde zenziwe kunye nokulungiswa kweenkqubo zokuvelisa.Ngokusekwa ngokukhawuleza kweqela lophando kunye nophuhliso, inkqubo yokuvelisa iproteinase K iye yoyiswa.Imveliso yenyanga ye powder eyomileyo ingaphezulu kwe-30 KG.Imveliso inomsebenzi ozinzile, umsebenzi ophezulu we-enzyme ethile, kwaye akukho mkhosi we-cytochrome kunye ne-nucleic acid residues.Wamkelekile kuqhagamshelwano lwe-XD BIOCHEM Fumana iphakheji yovavanyo (I-imeyile:sales@xdbiochem.comUmnxeba: +86 513 81163739).
XD BIOCHEM izisombululo zobugcisa ziquka
Ukusebenzisa ukuhlanganiswa kwe-plasmid yeekopi ezininzi, i-high-expression strains kunye nenqanaba lokubonisa le-8g / L likhethiwe, elinqoba ingxaki yezinga eliphantsi lokubonisa i-proteinase K.
Ngokusekwa kwenkqubo yokuhlanjululwa kwamanyathelo amaninzi, i-cytochrome yomkhosi kunye ne-nucleic acid i-residues ye-proteinase K isuswe ngempumelelo ngaphantsi kwexabiso eliqhelekileyo.
Ngovavanyo oluphezulu lwemixube yokhuselo lwebuffer, isithinteli esinokugcina ngokuzinzileyo iproteinase K kuma-37°C sikhethiwe.
Izithinteli zokuhlola ziyayoyisa ingxaki yokuba iproteinase K kulula ukuyidibanisa kwaye ihlike kwindawo eziphezulu, kwaye ibeka isiseko seproteinase K's isiqulatho esiqinileyo sokumisa-umkhenkce.
XD BIOCHEM proteinase K isampuli
Uvavanyo lokuzinza kwe-XD BIOCHEM proteinase K: akuyi kubakho tshintsho lubalulekileyo kumsebenzi emva kwe80 d kwiqondo lobushushu legumbi.
Uvavanyo lokuzinza kwe-XD BIOCHEM proteinase K: akuyi kubakho utshintsho olubalulekileyo kumsebenzi emva kwe-80 d kwiqondo lokushisa.
Ukuthelekiswa kwe-nucleic acid extraction effect ye-XD BIOCHEM proteinase K kunye neemveliso ezikhuphisanayo.Kwinkqubo ye-nucleic acid extraction, i-XD BIOCHEM kunye ne-proteinase K ekhuphisanayo isetyenziswa ngokulandelanayo.Ukusebenza kwe-extraction ye-XD BIOCHEM proteinase K iphezulu kwaye ixabiso le-Ct le-gene ekujoliswe kuyo liphantsi.
Ixesha lokuposa: Dec-31-2021