I-In vitro diagnostic reagent protease K39450-1-6 isetyenziswe ngokubanzi kwi-nucleic acid extraction.Ukutsalwa kwe-Nucleic acid linyathelo elisisiseko kuphando lwebhayoloji yemolekyuli, enokuthi ikhuphe i-DNA okanye i-RNA kwiiseli okanye izicubu ukunika isiseko sohlalutyo olulandelayo kunye novavanyo.
I-Protease K39450-1-6 yi-protease ekhethekileyo eneenkcukacha eziphezulu kunye nomsebenzi, onokudlala indima ebalulekileyo kwi-nucleic acid extraction.Oku kulandelayo kukusetyenziswa kweprotease K39450-1-6 kwi-nucleic acid extraction:
1. I-cell lysis: I-Protease K39450-1-6 inokuqhawula ngokufanelekileyo i-membrane zeseli kunye ne-nuclear membranes, ikhuphe i-DNA okanye i-RNA ngaphakathi kweeseli.Inokuthoba i-cell membrane lipids kunye neeprotheni, yonakalise ubume beseli, kwaye yenze lula ukukhupha ii-nucleic acids.
2. Ukucocwa kwe-Nucleic acid: I-Protease K39450-1-6 inokususa i-protein engcolileyo ngexesha lokukhutshwa kwe-nucleic acid.Kwinkqubo ye-nucleic acid extraction, kukho rhoqo iiprotheyini eziseleyo kwi-nucleic acid ekhutshwe, echaphazela uvavanyo olulandelayo kunye nohlalutyo.I-Protease K39450-1-6 inokuthoba ngokuthe ngqo iiprotheni, ngaloo ndlela isuse ezi ngcoliseko kunye nokuphucula ukucoceka kwe-nucleic acids.
3. I-Enzyme digestion reaction: I-Protease K39450-1-6 inokwenza i-enzyme yokugaya i-enzyme emva kokutsalwa kwe-nucleic acid.Ukwetyisa i-Enzyme bubuchule obudla ngokusetyenziswa kuphando lwebhayoloji yemolekyuli, enokusika i-DNA okanye i-RNA ibe ngamaqhekeza athile ukuze kuhlalutywe ngakumbi kunye nokulinga.Ubume obuphezulu kunye nomsebenzi we-protease K39450-1-6 yenza ukuba ibe lukhetho olufanelekileyo kwiimpendulo ze-enzyme yokugaya.
Isishwankathelo, i-protease K39450-1-6 inezicelo ezininzi kwi-nucleic acid extraction, njenge-cell lysis, i-nucleic acid yokuhlanjululwa kunye ne-enzyme digestion.Ukucaciswa kwayo okuphezulu kunye nomsebenzi kwenza kube sisixhobo esibalulekileyo kwinkqubo yokukhupha i-nucleic acid, enokuphucula ukucoceka kunye nomgangatho we-nucleic acids kunye nokubonelela ngesiseko esithembekileyo kwiimvavanyo ezilandelayo kunye nohlalutyo.
Ixesha lokuposa: Sep-28-2023