IIPIPE CAS: 5625-37-6 Ixabiso lomvelisi

I-PIPES (i-piperazine-1,4-bisethanesulfonic acid) yi-zwitterionic buffering ikhompawundi esetyenziswa ikakhulu kuphando lwebhayoloji kunye ne-biochemical.Ineempawu ezininzi ezibalulekileyo kunye nezicelo, kubandakanya:

I-agent ekhusela i-pH: I-PIPES sisithinteli esisebenzayo esinceda ukugcina uluhlu lwe-pH oluzinzileyo kwimifuniselo eyahlukeneyo yebhayoloji.Iqhele ukusetyenziswa kwimidiya yenkcubeko yeeseli, uvavanyo lwe-enzyme, kunye nokusetyenziswa kwebhayoloji yemolekyuli.

Umthamo ophakamileyo wokungathinteli: I-PIPES inomthamo ofanelekileyo wokuphazamisa ngaphakathi koluhlu lwe-pH ye-6.1 ukuya kwi-7.5, iyenza ifaneleke ukugcina iimeko ze-pH ezizinzileyo kuluhlu olubanzi lweenkqubo zebhayoloji.

Ukusebenzisana okuncinci kunye ne-biomolecules: I-PIPES iyaziwa ngokuphazamiseka kwayo okuphantsi kweenkqubo ze-biochemical kunye nokubophelela okuncinci kwiiprotheni kunye ne-enzymes, okwenza kube yinto efanelekileyo yokugcina ingqibelelo kunye nomsebenzi we-biomolecules.

Ifanelekile kwiimvavanyo ezixhomekeke kwiqondo lobushushu: I-PIPES iyakwazi ukugcina iipropathi zayo ze-buffering phezu koluhlu olubanzi lobushushu, kubandakanywa zombini ubushushu bomzimba kunye nokuphakama.Oku kuyenza ilungele imifuniselo efuna uzinzo kunye nokuchaneka kwiimeko ezahlukeneyo zobushushu.

Ukusetyenziswa kwe-Electrophoresis: I-PIPES isetyenziswa ngokuqhelekileyo njenge-buffer kwiindlela ze-gel electrophoresis, ezifana ne-RNA okanye i-DNA agarose gel electrophoresis, ngenxa yokufunxa kwayo okuphantsi kwe-UV kunye neempawu eziphezulu ze-conductivity.

Ukwenziwa kwechiza: I-PIPES ikwasetyenziswa ekuqulunqweni kwamayeza njenge-arhente ephazamisayo, ebonelela uzinzo kunye nokugcina eyona pH ilungileyo ukuze kusebenze ichiza.