I-2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2

I-2-Nitrophenyl-beta-D-glucopyranoside yi-chemical compound equkethe i-molecule ye-glucopyranoside ehlanganiswe neqela le-nitrophenyl.Ngokuqhelekileyo isetyenziswe njenge-substrate kwii-assays ze-enzymatic ukufumanisa kunye nokulinganisa umsebenzi wee-enzyme ezifana ne-beta-glucosidase.Iqela le-nitrophenyl linokucatshulwa yi-enzyme, okubangela ukukhululwa kwemveliso yombala ophuzi onokulinganiswa nge-spectrophotometrically.Le khompawundi iluncedo kakhulu ekufundeni i-enzyme kinetics kunye nokuhlolwa okuphezulu kwe-enzyme inhibitors okanye i-activators.Ikwasetyenziswa kuphando lwe-biochemical kuphando lwe-carbohydrate metabolism kunye ne-glycosidic-linkage-specific substrate.

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I-Enzyme substrate: I-ONPG iqhele ukusetyenziswa njenge-substrate ye-beta-galactosidase, i-enzyme eyenza i-hydrolyzes i-ONPG ukuvelisa ikhompawundi enombala otyheli (o-nitrophenol) enokubonwa lula nge-spectrophotometrically.Le mpendulo ye-enzymatic ingasetyenziselwa ukulinganisa umsebenzi we-beta-galactosidase, ukwenza i-ONPG ibe sisixhobo esixabisekileyo kwizifundo ze-enzymology.

Uvavanyo lwebhayoloji yeemolekyuli: I-ONPG isetyenziswa njengenxalenye yovavanyo lwebhayoloji yemolekyuli, ngakumbi kwiimvavanyo zofuzo zengxelo ye-beta-galactosidase.Kolu vavanyo, i-substrate esekelwe kwi-ONPG isetyenziselwa ukulinganisa umsebenzi we-reporter gene, edla ngokulawulwa ngumgqugquzeli othile wokulandelelana komdla.Umsebenzi we-beta-galactosidase, oboniswe ngotshintsho lombala oveliswe kwi-ONPG hydrolysis, inika ulwazi oluxabisekileyo malunga nomsebenzi womgqugquzeli.

Uhlalutyo lwentetho yemfuza: I-ONPG ikwasetyenziswa kuhlalutyo lwentetho yemfuza.Ngokudibanisa ulandelelwano lomgqugquzeli womdla kwi-beta-galactosidase gene, abaphandi banokulinganisa umsebenzi we-beta-galactosidase usebenzisa i-ONPG njenge-substrate.Umgangatho womsebenzi we-beta-galactosidase ubonakalisa amandla kunye nomsebenzi womgqugquzeli, ovumela ukuhlolwa kwamanqanaba e-gene expression.

Izicelo zokuxilonga: I-ONPG ingasetyenziswa kwizicelo zokuxilonga, ngakumbi ekubhaqweni kwe-pathogenic microorganisms.Iibhaktheriya ezahlukeneyo, ezifana ne-Escherichia coli kunye neentlobo ezithile ze-Shigella kunye ne-Salmonella, zivelisa i-beta-galactosidase enokuthi ihlukanise i-ONPG.Le mpendulo ye-hydrolysis ivelisa utshintsho olubonakalayo lombala, olunokusetyenziswa ukumisela ubukho bezi bhaktheriya kwiisampuli zeklinikhi.

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Iinkcukacha ezongezelelweyo:

Ukuqamba	C12H15NO8
Isivavanyi	99%
Imbonakalo	Umgubo otyheli okhanyayo
Inombolo yeCAS.	2816-24-2
Ukupakisha	Incinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Ugcino	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	ISO.