I-X-GAL CAS: 7240-90-6 Ixabiso lomenzi

Utshintsho lombala: I-X-Gal ayisoloko inombala kodwa, xa i-hydrolysis yi-β-galactosidase, ijika ibe luhlaza okwesibhakabhaka. Olu tshintsho lombala luvumela ukubonwa okubonakalayo kunye nokulinganiswa komsebenzi we-β-galactosidase.

Ukufunyanwa kweGene yeLacZ: I-X-Gal isetyenziselwa ukuchonga iiseli okanye izakhiwo ze-genetic ezibonisa i-gene ye-lacZ. I-LacZ isetyenziswa rhoqo njenge-reporter gene kwi-molecular biology ukuvavanya ukubonakaliswa kwe-gene okanye umsebenzi wokukhuthaza uphando.

Ukuhlolwa Kwekoloni: I-X-Gal isetyenziswa rhoqo kwiimvavanyo zokuhlolwa kwekoloni yebhaktheriya. Iikoloni zebhaktheriya eziveza i-LacZ zibonakala ziluhlaza okwesibhakabhaka xa zikhuliswe kwi-agar equlethe i-X-Gal, nto leyo eyenza kube lula ukuchongwa nokukhethwa kweekoloni ezine-lacZ.

Uhlalutyo lwe-Gene Fusion: I-X-Gal ikwasetyenziswa kwiimvavanyo ze-gene fusion. Xa i-gene ekujoliswe kuyo idibene ne-lacZ gene, i-X-Gal staining ingatyhila ipateni yokubonakaliswa kweproteni ye-fusion ngaphakathi kwiseli okanye kwizicubu.

Indawo yeProteni: Ukufaka idayi ye-X-Gal kungasetyenziselwa ukuphanda indawo yeproteni engaphantsi kweseli. Ngokudibanisa iproteni enomdla kwi-lacZ gene, umsebenzi we-β-galactosidase unokubonisa apho iproteni ifumaneka khona ngaphakathi kwiseli.

Ii-X-Gal Analogues: Iintlobo ze-X-Gal eziguquliweyo, ezifana neBluo-Gal okanye iRed-Gal, ziye zaphuhliswa ukuvumela ezinye iindlela zokuphuhlisa imibala. Ezi analogues zenza umahluko phakathi kweeseli okanye izicubu ze-lacZ-positive kunye ne-lacZ-negative usebenzisa imibala eyahlukeneyo.