I-2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2

I-substrate ye-enzyme: I-ONPG isetyenziswa kakhulu njenge-substrate ye-beta-galactosidase, i-enzyme e-hydrolyzes i-ONPG ukuvelisa i-compound enombala otyheli (i-o-nitrophenol) enokubonwa ngokulula nge-spectrophotometric. Le mpendulo ye-enzyme ingasetyenziselwa ukulinganisa umsebenzi we-beta-galactosidase, nto leyo eyenza i-ONPG ibe sisixhobo esibalulekileyo kwizifundo ze-enzymology.

Uvavanyo lwebhayoloji yemolekyuli: I-ONPG isetyenziswa njengesiseko kwiimvavanyo ezahlukeneyo zebhayoloji yemolekyuli, ngakumbi kwiimvavanyo ze-beta-galactosidase reporter gene. Kule mvavanyo, isiseko esisekelwe kwi-ONPG sisetyenziselwa ukulinganisa umsebenzi we-reporter gene, odla ngokulawulwa lulandelelwano oluthile lomdla we-promoter. Umsebenzi we-beta-galactosidase, oboniswa lutshintsho lombala oluveliswa kwi-hydrolysis ye-ONPG, unika ulwazi oluxabisekileyo malunga nomsebenzi we-promoter.

Uhlalutyo lokubonakaliswa kwezakhi zofuzo: I-ONPG ikwasetyenziswa kuhlalutyo lokubonakaliswa kwezakhi zofuzo. Ngokudibanisa ulandelelwano lomdla wokukhuthaza kunye ne-beta-galactosidase gene, abaphandi banokulinganisa umsebenzi we-beta-galactosidase besebenzisa i-ONPG njengesiseko. Inqanaba lomsebenzi we-beta-galactosidase libonakalisa amandla kunye nomsebenzi womxhasi, okuvumela uvavanyo lwamanqanaba okubonakaliswa kwezakhi zofuzo.

Ukusetyenziswa kokuxilongwa: I-ONPG ingasetyenziswa kwiinkqubo zokuxilonga, ingakumbi ekufumaneni iintsholongwane ezibangela izifo. Iintsholongwane ezahlukeneyo, ezifana ne-Escherichia coli kunye neentlobo ezithile zeShigella kunye neSalmonella, zivelisa i-beta-galactosidase enokususa i-ONPG. Le mpendulo ye-hydrolysis ivelisa utshintsho olubonakalayo lombala, olunokusetyenziswa ukufumanisa ubukho bezi ntsholongwane kwiisampuli zeklinikhi.