3-[(3-Cholanidopropyl)dimethylammonio]-1-propanesulfonate CAS:75621-03-3

I-CHAPS (3--[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate) sisicoci esisetyenziswa ngokuqhelekileyo kwi-biochemistry kunye ne-molecular biology.Sisicoci se-zwitterionic, okuthetha ukuba sineqela elichasa kakuhle nelibi.

I-CHAPS yaziwa ngokukwazi ukunyibilikisa kunye nokuzinzisa iiproteni ze-membrane, iyenza ibe luncedo kwiinkqubo ezahlukeneyo ezinje ngokutsalwa kweprotein, ukucocwa, kunye nokulinganisa iimpawu.Iphazamisa ukusebenzisana kwe-lipid-protein, ivumela iiprotheni ze-membrane ukuba zikhutshwe kwilizwe labo lokuzalwa.

Ngokungafaniyo nezinye izicoci, i-CHAPS ithambile kwaye ayidibanisi iiprotheyini ezininzi, iyenza ibe lolona khetho lubalaseleyo lokugcina ulwakhiwo lweprotheyini kunye nokusebenza ngexesha lovavanyo.Inokunceda nokuthintela ukudityaniswa kweeprotheyini.

I-CHAPS iqhele ukusetyenziswa kubuchule obufana ne-SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), ugxininiso lwe-isoelectric, kunye ne-Western blotting.Ikwasetyenziswa rhoqo kwizifundo ezibandakanya i-enzymes ebotshwe kwi-membrane, ukuhanjiswa komqondiso, kunye nokusebenzisana kweprotein-lipid.

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Iinkcukacha zeMveliso

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Isicelo kunye nesiphumo

Ukutsalwa kweProtheyini: ICHAPS iqhele ukusetyenziswa ukukhupha iiproteni zenwebu kwiisampulu zebhayoloji.Inceda ukunyibilikisa ezi proteni kunye nokugcina ubume bazo bendalo.

Ukucocwa kweeProtheyini: I-CHAPS isetyenziswa kwiindlela ezahlukeneyo zokucoca iiprotheyini, ezifana ne-affinity chromatography.Inokongezwa kwii-buffers zokuhlanjululwa ukuze i-solubilize kwaye izinzile iiprotheni ze-membrane ngexesha lenkqubo yokucoca.

Ubume beProtein: I-CHAPS isoloko isetyenziswa kwizifundo ezibandakanya ukubonakaliswa kweeproteni zenwebu.Inceda ukugcina ubume beprotheyini kunye nokusebenza ngexesha leenkqubo zovavanyo ezifana novavanyo lomsebenzi we-enzyme, ukunxibelelana kweeprotheyini, kunye nohlalutyo lwe-spectroscopic.

Izifundo zeMembrane Protein: Iiprotheyini zeMembrane zidlala indima ebalulekileyo kwiinkqubo ezininzi zeselula.I-CHAPS idla ngokusetyenziswa kuphando olunxulumene nokuhanjiswa komqondiso, ukusebenza kwetshaneli ye-ion, ukusebenzisana kweprotein-lipid, kunye ne-membrane protein crystallization.

I-Electrophoresis: I-CHAPS isetyenziswa kubuchule obufana ne-SDS-PAGE kunye ne-isoelectric egxininisa ukunyibilikisa iiproteni ze-membrane kunye nokuququzelela ukwahlukana kunye nohlalutyo.

Ukupakishwa kweMveliso:

Iinkcukacha ezongezelelweyo:

Ukuqamba	I-C32H58N2O7S
Isivavanyi	99%
Imbonakalo	Umgubo omhlophe wekristale
Inombolo yeCAS.	75621-03-3
Ukupakisha	Incinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Ugcino	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	ISO.