3-[(3-Cholanidopropyl)dimethylammonio]-1-propanesulfonate CAS:75621-03-3 Umfanekiso Obonakalayo

I-3-[(3-Cholanidopropyl)dimethylammonio]-1-propanesulfonate CAS:75621-03-3

I-CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate) sisicoci esisetyenziswa kakhulu kwi-biochemistry nakwi-molecular biology. Sisicoci se-zwitterionic, oko kuthetha ukuba sineqela eline-positive ne-negative charge.

I-CHAPS yaziwa ngokukwazi kwayo ukunyibilikisa nokuzinzisa iiproteni ze-membrane, nto leyo eyenza ukuba ibe luncedo kwiindlela ezahlukeneyo ezifana nokukhupha iiproteni, ukucoca, kunye nokuchonga iimpawu. Iphazamisa ukusebenzisana kwe-lipid-protein, ivumela iiproteni ze-membrane ukuba zikhutshwe kwimeko yazo yendalo.

Ngokungafaniyo nezinye izicoci, i-CHAPS ithambile kwaye ayiguquli iiproteni ezininzi, nto leyo eyenza ukuba ibe lukhetho oluhle kakhulu lokugcina ulwakhiwo lweproteni kunye nokusebenza kwayo ngexesha lovavanyo. Inokunceda ukuthintela ukuhlangana kweeproteni.

I-CHAPS isetyenziswa kakhulu kwiindlela ezifana ne-SDS-PAGE (i-sodium dodecyl sulfate polyacrylamide gel electrophoresis), i-isoelectric focusing, kunye ne-Western blotting. Ikwasetyenziswa rhoqo kwizifundo ezibandakanya ii-enzymes ezibotshelelwe kwi-membrane, i-signal transduction, kunye nokusebenzisana kwe-protein-lipid.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Ukutsalwa kweeProteni: I-CHAPS isetyenziswa kakhulu ekutsaleni iiproteni ze-membrane kwiisampuli zebhayoloji. Inceda ekunyibilikiseni ezi proteni nokugcina ulwakhiwo lwazo lwendalo.

Ukucocwa kweeProteni: I-CHAPS isetyenziswa kwiindlela ezahlukeneyo zokucocwa kweeproteni, ezifana ne-affinity chromatography. Ingongezwa kwii-purification buffers ukuze inyibilikise kwaye izinzise iiproteni ze-membrane ngexesha lenkqubo yokucocwa.

Ukuchazwa kweeProteni: I-CHAPS idla ngokusetyenziswa kwizifundo ezibandakanya ukuchazwa kweeproteni ze-membrane. Inceda ukugcina ulwakhiwo lweproteni kunye nokusebenza kwayo ngexesha leenkqubo zovavanyo ezifana novavanyo lomsebenzi we-enzyme, ukusebenzisana kweproteni kunye neproteni, kunye nohlalutyo lwe-spectroscopic.

Izifundo zeProtein yeMembrane: Iiprotein zeMembrane zidlala indima ebalulekileyo kwiinkqubo ezininzi zeseli. I-CHAPS isetyenziswa rhoqo kuphando olunxulumene nokudluliselwa kwesignali, umsebenzi wetshaneli ye-ion, ukusebenzisana kweproteni-lipid, kunye nokukristaleka kweproteni yemembrane.

I-Electrophoresis: I-CHAPS isetyenziswa kwiindlela ezifana ne-SDS-PAGE kunye ne-isoelectric egxile ekunyibilikiseni iiproteni ze-membrane kunye nokwenza kube lula ukwahlukana kwazo kunye nohlalutyo.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C32H58N2O7S
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe wekristale
Inombolo ye-CAS	75621-03-3
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.