I-5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS:4264-82-8

I-5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide yikhompawundi esetyenziswa kwizifundo ezahlukeneyo ze-biochemical, ngokukodwa ukufumanisa kunye nokubonwa komsebenzi we-enzyme.I-substrate enokuthi ifakwe i-hydrolyzed ngama-enzymes athile, okukhokelela ekukhululweni kwemveliso enemibala okanye ye-fluorescent.

Le khompawundi isetyenziswa ngokuqhelekileyo kwiimvavanyo zokubona ubukho kunye nomsebenzi wee-enzyme ezifana ne-beta-galactosidase kunye ne-beta-glucuronidase.Ezi enzymes ziqhekeza amaqela e-acetyl kunye ne-glucosaminide ukusuka kwi-substrate, ekhokelela ekubunjweni kwechromophore eluhlaza okwesibhakabhaka okanye eluhlaza.

Isakhiwo esiyingqayizivele se-5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide ivumela ukufumanisa lula kunye nokulinganisa umsebenzi we-enzyme.Ukusetyenziswa kwayo kwiindlela ezahlukeneyo zokulinga, kubandakanywa i-histochemistry, i-immunohistochemistry, kunye ne-cell-based assays, ibe negalelo ekuqondeni ngcono imisebenzi ye-enzyme.

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I-5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-Gluc) i-substrate ye-chromogenic esetyenziswa ngokuqhelekileyo ukufumanisa umsebenzi we-beta-glucuronidase (GUS).I-GUS yi-enzyme efumaneka kwizinto ezahlukeneyo eziphilayo, kuquka iibhaktheriya, izityalo kunye nezilwanyana ezincancisayo.I-X-Gluc isetyenziswa rhoqo kwiimvavanyo zeentatheli ze-GUS kunye nophando lwebhayoloji yemolekyuli.

Ukusetyenziswa okuphambili kwe-X-Gluc kwi-histochemical staining techniques, apho inokubona imbonakalo kunye nomsebenzi we-enzyme ye-GUS.Le substrate i-cell-permeable kwaye iba yi-hydrolyzed yi-GUS, okukhokelela ekwenziweni kwe-precipitate eluhlaza okwesibhakabhaka okanye imveliso enganyibilikiyo.Oku kuluhlaza okwesibhakabhaka kuvumela abaphandi ukuba bachonge kwaye bafake umsebenzi we-GUS kwiiseli, izicubu, kunye nezinto ezipheleleyo.

I-X-Gluc inokusetyenziswa kwiimvavanyo zobungakanani ukulinganisa umsebenzi we-enzyme ye-GUS.Ukuqina kombala oluhlaza okwesibhakabhaka okanye ubungakanani bemveliso eyenziweyo inokudityaniswa nenqanaba lokubonakaliswa kwe-GUS okanye umsebenzi wayo we-enzymatic.

Ukongeza, i-X-Gluc isetyenzisiwe kuphando lwemfuza yezityalo ukufunda ukubonakaliswa kofuzo, umsebenzi wokukhuthaza, kunye notshintsho lwezityalo.Ikwasetyenziswe kwiinkqubo zebhaktheriya zokubumba kunye nokufumanisa iiprotheni ze-GUS fusion.

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Iinkcukacha ezongezelelweyo:

Ukuqamba	I-C16H18BrClN2O6
Isivavanyi	99%
Imbonakalo	Umgubo omhlophe
Inombolo yeCAS.	4264-82-8
Ukupakisha	Incinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Ugcino	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	ISO.