I-HEPES CAS: 7365-45-9 Ixabiso lomenzi

I-HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid) sisithinteli sebhayoloji esisetyenziswa kakhulu kuphando lwebhayoloji kunye neekhemikhali. Yaziwa ngokukwazi kwayo ukugcina i-pH ezinzileyo kwizisombululo zamanzi, okwenza ifaneleke kwiintlobo ngeentlobo zezicelo ezibandakanya iinkqubo zeseli kunye ne-enzymatic. I-HEPES yikhompawundi ye-zwitterionic, oko kuthetha ukuba ineentlawulo ezilungileyo nezingalunganga kumaqela ahlukeneyo asebenzayo, nto leyo eyivumela ukuba imelane notshintsho kwi-pH olubangelwa kukongezwa kwee-asidi okanye iziseko. Isetyenziswa rhoqo kwinkcubeko yeseli, uvavanyo lwee-enzyme, izifundo zeproteni, iimvavanyo ze-electrophoresis, kunye nefomyula yamayeza.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Ukuthintela i-pH: I-HEPES isetyenziswa kakhulu njengesixhobo sokuthintela ukugcina i-pH ezinzileyo kwi-cell culture media kunye novavanyo lwebhayoloji. Isebenza ngempumelelo kuluhlu lwe-pH ye-physiological ukusuka kwi-6.8 ukuya kwi-8.2, okwenza ifaneleke ukugcina iimeko ezifanelekileyo zeenkqubo zebhayoloji.

Inkcubeko yeeseli: I-HEPES idla ngokufakwa kwi-cell culture media ukuze kuzinziswe inqanaba le-pH, ibonelela ngendawo efanelekileyo yokukhula kweeseli kunye nokugcina ubomi beeseli buhlala buhleli. Inceda ekuthinteleni ukuguquguquka kwe-pH okunokuchaphazela kakubi ukuziphatha kweeseli kunye neziphumo zovavanyo.

Uvavanyo lwe-enzyme: I-HEPES idla ngokusetyenziswa njenge-buffer kwi-enzyme reactions ngenxa yokuba ikwazi ukugcina i-pH ethile. Inceda ekugcineni umsebenzi we-enzyme ofanelekileyo kunye nozinzo, ngaloo ndlela iphucula ukuchaneka kunye nokuthembeka kovavanyo lwe-enzyme.

Izifundo zeproteni: I-HEPES isetyenziswa kwiimvavanyo ezahlukeneyo ezinxulumene neproteni, kubandakanya ukucocwa kweproteni, ukwakheka kweproteni, kunye nohlalutyo lwesakhiwo seproteni. Inceda ukuzinzisa i-pH kunye nokugcina ukugoba kunye nozinzo olufanelekileyo lweproteni ngexesha lezi mvavanyo.

I-Electrophoresis: I-HEPES ifumana ukusetyenziswa kwiindlela ze-gel electrophoresis, ezifana ne-SDS-PAGE kunye ne-agarose gel electrophoresis. Isetyenziswa njenge-arhente yokukhusela kwi-buffer esebenzayo, inika imeko-bume ye-pH ezinzileyo yokwahlula kunye nohlalutyo lwee-biomolecules.

Iifomyula zamayeza: I-HEPES isetyenziswa ekwenzeni iimveliso zamayeza kunye ne-biotechnology. Inceda ukugcina uzinzo kunye nokuthembeka kwezithako ezisebenzayo, ukuqinisekisa ukusebenza kwazo kunye nexesha lazo lokuphelelwa.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C8H18N2O4S
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	7365-45-9
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.