I-Ethidium Bromide CAS:1239-45-8

Ukubona ngeliso le-DNA/RNA: I-EtBr isetyenziswa kakhulu ukubonisa ngeliso lengqondo i-DNA kunye ne-RNA kwi-agarose gel electrophoresis. Ihlangana phakathi kwezibini ezisisiseko zeemolekyuli ze-nucleic acid, nto leyo ebangela ukuba zikhanye xa zikhanyiswe kukukhanya kwe-UV. Oku kuvumela abaphandi ukuba babone ngeliso lengqondo kwaye bahlalutye ukwahlukana kweziqwenga ze-DNA okanye ze-RNA ngokusekelwe kubukhulu okanye ulandelelwano lwazo.

Ukudaya kwe-DNA: I-EtBr ingasetyenziselwa ukudaya i-DNA kwiinkqubo ezahlukeneyo zovavanyo. Ingongezwa kwiisampulu ze-DNA, ezifana nee-plasmids ezicociweyo okanye iimveliso ze-PCR, ukuqinisekisa ubukho bazo kunye noxinzelelo lwazo. I-DNA edayayo ingabonwa kusetyenziswa ukukhanya kwe-UV okanye inkqubo ye-gel imaging.

Ubungakanani beDNA: I-EtBr ingasetyenziselwa ukubala ubungakanani beDNA. Ngokuthelekisa ubunzulu beebhendi zeDNA ezinemibala ye-EtBr kwijeli ye-agarose kunye nemigangatho eyaziwayo yobungakanani beDNA, ubungakanani beDNA obuqikelelweyo kwisampulu bunokufunyanwa.

I-Fluorescent Microscopy: I-EtBr ingasetyenziswa kwi-fluorescence microscopy ukuze ifake ibala kwaye ibone i-DNA kwiiseli eziphilayo okanye kwizicubu. Ivumela abaphandi ukuba bafunde indawo ye-DNA kunye ne-dynamics ngaphakathi kweeseli okanye bajonge ukuqhekeka kwe-DNA kwiiseli ze-apoptotic.