I-Diethylene triamine pentaacetic acid CAS:67-43-6

I-Diethylene triamine pentaacetic acid (DTPA) yikhemikhali esetyenziswa kakhulu njenge-chelating agent. Inamandla okubopha ngee-ion zesinyithi esinzima, ngakumbi ezo zifana ne-lead, i-mercury, kunye ne-cadmium, ukuze zenze ii-complexes ezizinzileyo. I-DTPA isetyenziswa kwiindlela ezahlukeneyo zokusebenza, kubandakanya ukulungiswa kwendalo, unyango lwezonyango, iinkqubo zoshishino, kunye ne-radiopharmaceuticals. Iimpawu zayo zesinyithi-chelating zenza ukuba isebenze ekususeni iintsimbi ezinzima emanzini amdaka, ekunyangeni ityhefu yesinyithi esinzima, ekuthintela ukuphazamiseka kwiimpendulo zeekhemikhali, kunye nokwenza kube lula ukuhanjiswa kwamayeza onyango ekujoliswe kuwo. Ngokubanzi, i-DTPA yikhemikhali eguquguqukayo enezicelo ezibalulekileyo kumashishini ahlukeneyo.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Ukungasebenzi kwee-RNases: Injongo ephambili ye-DEPC kukungasebenzi kwee-RNases, ezizi-enzymes ezinokonakalisa i-RNA. I-DEPC isabela kunye neentsalela ze-histidine kwii-RNases, iguqula iindawo zazo ezisebenzayo kwaye izenze zingasebenzi. Oku kuqinisekisa ukugcinwa kobunyani be-RNA ngexesha lovavanyo olufana nokwahlulwa kwe-RNA, ukubhalwa kwe-reverse transcription, kunye nokulandelelana kwe-RNA.

Ukugcinwa kweesampuli ze-RNA: Unyango lwe-DEPC ludla ngokusetyenziswa kwizixhobo zelebhu, ezifana neetyhubhu ze-microcentrifuge, iincam zepipette, kunye namanzi asetyenziswa kuvavanyo lwe-RNA. Oku kunceda ukuthintela naziphi na ii-RNases eziseleyo ezinokubakho kwaye kuthintele ukonakala kwe-RNA ngexesha lokuphathwa nokugcinwa kwesampuli.

Ukulungiswa kwee-reagent ezingenayo i-RNAse: I-DEPC ingasetyenziselwa ukunyanga izisombululo okanye ii-reagents ekufuneka zingenazo i-RNAse, ezifana nee-buffers, izisombululo ze-Tris-EDTA (TE), okanye amanzi asetyenziswa kuvavanyo lwebhayoloji yemolekyuli. Ezi zisombululo zinyangiweyo zingasetyenziswa ngokukhuselekileyo ukunciphisa ukonakala kwe-RNA.

Ukuguqulwa kwe-DNA: I-DEPC isenokusabela namaqela e-amino kwiimolekyuli ze-DNA, nto leyo ekhokelela ekuguqulweni kweziseko ze-DNA nge-carbethoxylated. Oku kungasetyenziswa kwizicelo ezithile, ezifana nokulandelelana kwe-DNA okanye ukuguqula i-DNA kwizifundo zokudibanisa.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C6H10O5
Uvavanyo	99%
Inkangeleko	Ulwelo olungenambala
Inombolo ye-CAS	1609-47-8
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.