I-DEPC CAS:1609-47-8 Ixabiso lomenzi

I-Diethyl pyrocarbonate (DEPC) yikhemikhali esetyenziswa kakhulu kwiilebhu zebhayoloji yemolekyuli. Lulwelo olucacileyo nolungenambala olusabela kakhulu kwiikhompawundi ze-nucleophilic ezifana nama-amine kunye nama-thiol. I-DEPC isetyenziselwa ikakhulu ukuvala ii-RNases (ribonucleases), ii-enzymes ezinokonakalisa i-RNA, ngokuguqula iindawo zazo ezisebenzayo. Ifezekisa oku ngokusabela kwiintsalela ze-histidine kwi-enzyme, ezibalulekileyo kumsebenzi wayo. Oku kwenza i-DEPC ibe sisixhobo esibalulekileyo kwiimvavanyo ezinxulumene ne-RNA kwaye iqinisekisa ukugcinwa kobunyani be-RNA. Ukongeza kwiipropati zayo zokuvala ii-RNase, i-DEPC inokutshintsha nezinye ii-biomolecules, ezifana neeproteni kunye ne-DNA, ngenxa yokusebenza kwayo namaqela e-nucleophilic. Kufuneka kuqatshelwe xa kusetyenziswa i-DEPC, njengoko inetyhefu kwaye inokuba ne-mutagenic.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Ukungasebenzi kwee-RNases: Injongo ephambili ye-DEPC kukungasebenzi kwee-RNases, ezizi-enzymes ezinokonakalisa i-RNA. I-DEPC isabela kunye neentsalela ze-histidine kwii-RNases, iguqula iindawo zazo ezisebenzayo kwaye izenze zingasebenzi. Oku kuqinisekisa ukugcinwa kobunyani be-RNA ngexesha lovavanyo olufana nokwahlulwa kwe-RNA, ukubhalwa kwe-reverse transcription, kunye nokulandelelana kwe-RNA.

Ukugcinwa kweesampuli ze-RNA: Unyango lwe-DEPC ludla ngokusetyenziswa kwizixhobo zelebhu, ezifana neetyhubhu ze-microcentrifuge, iincam zepipette, kunye namanzi asetyenziswa kuvavanyo lwe-RNA. Oku kunceda ukuthintela naziphi na ii-RNases eziseleyo ezinokubakho kwaye kuthintele ukonakala kwe-RNA ngexesha lokuphathwa nokugcinwa kwesampuli.

Ukulungiswa kwee-reagent ezingenayo i-RNAse: I-DEPC ingasetyenziselwa ukunyanga izisombululo okanye ii-reagents ekufuneka zingenazo i-RNAse, ezifana nee-buffers, izisombululo ze-Tris-EDTA (TE), okanye amanzi asetyenziswa kuvavanyo lwebhayoloji yemolekyuli. Ezi zisombululo zinyangiweyo zingasetyenziswa ngokukhuselekileyo ukunciphisa ukonakala kwe-RNA.

Ukuguqulwa kwe-DNA: I-DEPC isenokusabela namaqela e-amino kwiimolekyuli ze-DNA, nto leyo ekhokelela ekuguqulweni kweziseko ze-DNA nge-carbethoxylated. Oku kungasetyenziswa kwizicelo ezithile, ezifana nokulandelelana kwe-DNA okanye ukuguqula i-DNA kwizifundo zokudibanisa.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C6H10O5
Uvavanyo	99%
Inkangeleko	Ulwelo olungenambala
Inombolo ye-CAS	1609-47-8
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.