HEPES CAS: 7365-45-9 Ixabiso lomvelisi

i-pH buffering: I-HEPES isetyenziswa ngokubanzi njenge-arhente ekhuselayo ukugcina i-pH ezinzileyo kumajelo eendaba enkcubeko yeeseli kunye novavanyo lwebhayoloji.Isebenza ngokufanelekileyo kwi-pH ye-physiological pH ye-6.8 ukuya kwi-8.2, eyenza ifaneleke ukugcina iimeko ezifanelekileyo kwiinkqubo zezinto eziphilayo.

Inkcubeko yeeseli: I-HEPES yongezwa ngokuqhelekileyo kwimidiya yenkcubeko yeseli ukuzinzisa inqanaba le-pH, ukubonelela ngendawo efanelekileyo yokukhula kweeseli kunye nokugcina ukusebenza kweeseli.Kuyanceda ukunqanda ukuguquguquka kwe-pH okunokuthi kube nefuthe elibi kwindlela yokuziphatha kweeseli kunye neziphumo zovavanyo.

Uvavanyo lwe-enzyme: I-HEPES isoloko isetyenziswa njengesithinteli kwiintshukumo ze-enzymatic ngenxa yokukwazi kwayo ukugcina i-pH ethile.Inceda ekugcineni umsebenzi ofanelekileyo we-enzymatic kunye nokuzinza, ngaloo ndlela uphucula ukuchaneka kunye nokuthembeka kovavanyo lwe-enzyme.

Izifundo zeprotheyini: I-HEPES iqeshwe kwiimvavanyo ezahlukeneyo ezinxulumene neprotheni, kubandakanywa ukuhlanjululwa kweeprotheyini, i-protein crystallization, kunye nohlalutyo lwesakhiwo seprotheni.Inceda ukuzinzisa i-pH kunye nokugcina iprotheyini efanelekileyo yokugoqa kunye nokuzinza ngexesha lovavanyo.

I-Electrophoresis: I-HEPES ifumana isicelo kwiindlela ze-gel electrophoresis, ezifana ne-SDS-PAGE kunye ne-agarose gel electrophoresis.Isetyenziswe njenge-agent ephazamisayo kwi-buffer esebenzayo, inika indawo ezinzileyo ye-pH yokwahlula kunye nohlalutyo lwee-biomolecules.

Ukwenziwa kwamachiza: I-HEPES isetyenziswa ekuqulunqweni kweemveliso zamachiza kunye ne-biotechnological.Inceda ukugcina ukuzinza kunye nokunyaniseka kwezithako ezisebenzayo, ukuqinisekisa ukusebenza kwazo kunye nobomi beshelufu.