I-Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside CAS:24404-53-3

I-Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside isetyenziswa kakhulu njengesiseko se-enzyme i-β-galactosidase. Xa i-β-galactosidase isebenza kuyo, le compound iyanyibilika, nto leyo ebangela ukuveliswa kwe-p-nitrophenol okanye i-o-nitrophenol, enombala otyheli onokubonwa lula. Oku kuyenza ibe luncedo njengesiseko see-assays ezilinganisa umsebenzi we-β-galactosidase.

Enye indlela eqhelekileyo yokusebenzisa iPhenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ikuvavanyo lwe-reporter gene. Kolu vavanyo, i-gene enomdla idityaniswe ne-reporter gene, njenge-beta-galactosidase gene. Umsebenzi we-reporter gene ujongwa ngokulinganisa umsebenzi we-β-galactosidase kusetyenziswa i-Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside njenge-substrate. Oku kuvumela abaphandi ukuba bafunde ukubonakaliswa kwe-gene kunye nokulawulwa kwayo.

I-Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ingasetyenziselwa ukuvavanya izithinteli okanye izivuseleli ze-β-galactosidase. Ngokuvavanya iikhompawundi ezahlukeneyo okanye abaviwa beziyobisi, abaphandi banokuchonga iimolekyuli ezilawula umsebenzi wale enzyme, ezinokuba nezicelo zonyango kwizifo okanye kwiimeko ezithile.

Ukongeza, le khompawundi ingasetyenziswa ekucoceni iiproteni. I-β-galactosidase idla ngokudityaniswa neeproteni ezinomdla ukuze kube lula ukuzibona nokuzicoca. I-Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ingasetyenziswa njengesiseko sokuhlola nokucoca ezi proteni ze-fusion ngokufumanisa umsebenzi we-β-galactosidase.