IMIbhobho yetyuwa ye-monosodium CAS: 10010-67-0

I-Agent eBhabhulayo: I-HEPES-Na isetyenziswa ikakhulu njenge-agent ephazamisayo ukugcina uluhlu lwe-pH oluzinzileyo kwimifuniselo yebhayoloji kunye neyekhemikhali.Iyakwazi ukumelana ngempumelelo neenguqu ze-pH ezibangelwa ukongezwa kwe-acids okanye iziseko.

INkcubeko yeSeli: I-HEPES-Na isoloko yongezwa kwimidiya yenkcubeko yeseli ukubonelela nge-pH ezinzileyo kunye neyona ndlela ifanelekileyo yokukhula kweeseli kunye nokusebenza.Inceda ukuchasana nokuguquguquka kwe-pH okwenzekayo ngenxa yeenkqubo zemetabolism yeeseli eziphilayo.

Uvavanyo lwe-Enzyme: I-HEPES-Na iqhele ukusetyenziswa njenge-buffer kwiimvavanyo ze-enzyme.Inceda ukugcina i-pH kwinqanaba eliphezulu lomsebenzi we-enzyme kunye nokuzinza, ukuqinisekisa iziphumo ezichanekileyo nezithembekileyo.

Ubuchwephesha bebhayoloji yeMolekyuli: I-HEPES-Na isetyenziswa ngokubanzi kwiindlela ezahlukeneyo zebhayoloji yemolekyuli, njenge-DNA kunye ne-RNA yodwa, ukukhulisa i-PCR, kunye nohlalutyo lweprotheyini.Inceda ukugcina iimeko ze-pH ezizinzileyo ngexesha lezi nkqubo, eziyimfuneko ekugcineni ingqibelelo kunye nokusebenza kweeathomu zebhayoloji.

I-Electrophoresis: Kwi-gel electrophoresis, i-HEPES-Na isetyenziswe njenge-buffer ukubonelela indawo ezinzileyo ye-pH yokwahlula i-DNA, i-RNA, kunye neeprotheni.Inceda ukuqinisekisa ukufuduka okufanelekileyo kunye nesisombululo seemolekyuli ngaphakathi kwe-matrix ye-gel.