Ityuwa yesodium ye-5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide CAS:129541-41-9

Ityuwa yesodium ye-5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide yikhemikhali esetyenziswa kakhulu kuphando lwelabhoratri kunye nokuxilongwa. Idla ngokubizwa ngokuba yi-X-Gluc kwaye isetyenziswa kakhulu njengesiseko sokubona umsebenzi we-enzyme ye-beta-glucuronidase.

Xa i-beta-glucuronidase ikhona, iyawuqhawula umxokelelwane we-glucuronide kwi-X-Gluc, nto leyo ephumela ekukhululweni kwedayi eluhlaza okwesibhakabhaka ebizwa ngokuba yi-5-bromo-4-chloro-3-indolyl. Le mpendulo idla ngokusetyenziselwa ukubona ngokubonakalayo okanye nge-spectrophotometric ukubonakaliswa kwe-enzyme ye-beta-glucuronidase kwiiseli okanye kwizicubu.

Uhlobo lwetyuwa yesodiyam ye-X-Gluc luphucula ukunyibilika kwayo kwizisombululo zamanzi, nto leyo eyenza kube lula ukusetyenziswa kwayo kwiimvavanyo zelebhu. I-X-Gluc isetyenziswa kakhulu kuphando lwebhayoloji yeemolekyuli ukufunda ukubonakaliswa kwezakhi zofuzo, umsebenzi wokukhuthaza, kunye novavanyo lwezakhi zofuzo ze-reporter. Ingasetyenziselwa nokufumanisa ubukho bezinto eziphilayo ezivelisa i-beta-glucuronidase, ezifana neebhaktheriya ezithile, kwizifundo ze-microbiological.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Ukufunyanwa kweGUS: I-X-Gluc iqhekeka yi-enzyme yeGUS ibe yi-compound eluhlaza okwesibhakabhaka enganyibilikiyo eyaziwa ngokuba yi-5-bromo-4-chloro-3-indole (X-Ind). Le mpendulo ivumela ukubonakala kunye nokulinganisa umsebenzi weGUS kwiiseli kunye nezicubu.

Izifundo zokubonakaliswa kwezakhi zofuzo: I-X-Gluc isetyenziswa njengemolekyuli yengxelo kwizifundo zokubonakaliswa kwezakhi zofuzo. Ngokudibanisa i-GUS gene kwipromoter enomdla, abaphandi banokumisela umsebenzi kunye nepatheni yokubonakaliswa kwendawo kunye nexesha yompromoter ngokubona umsebenzi we-GUS kusetyenziswa i-X-Gluc.

Uhlalutyo lwezityalo eziguqulweyo: Inkqubo ye-GUS reporter gene isetyenziswa kakhulu kwi-biology ye-molecular yezityalo. Ukupenda i-X-Gluc kuvumela abaphandi ukuba babone kwaye bafunde iipatheni zokubonakaliswa kwe-transgene kwizityalo. Oku kunceda ekuqondeni ukulawulwa kwe-gene, ukubonakaliswa kwezicubu ezithile, kunye ne-biology yophuhliso kwizityalo.

Ubunjineli bemfuza: I-X-Gluc isetyenziswa njengophawu olukhethiweyo kuvavanyo lobunjineli bemfuza. Ngokudibanisa i-GUS gene ne-gene yangaphandle enomdla, ukudaywa kwe-X-Gluc kungasetyenziselwa ukuchonga utshintsho oluphumelelayo kunye nokuhlanganiswa kwemfuza efunekayo kwi-organism.

Uphando lwe-microbiology: I-X-Gluc ingasetyenziselwa ukufumanisa nokuchonga iibhaktheriya ezivelisa i-GUS. I-enzyme i-GUS ifumaneka kwiintlobo ezahlukeneyo zeebhaktheriya, kwaye ukudaywa kwe-X-Gluc kuvumela ukubonakala nokuchonga iibhaktheriya ezine-GUS kwizifundo ze-microbiology.

Isampuli yeMveliso

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C14H14BrClNNaO7
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	129541-41-9
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.