I-5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide yikhompawundi esetyenziswa kwizifundo ezahlukeneyo ze-biochemical, ngokukodwa ukufumanisa kunye nokubonwa komsebenzi we-enzyme.I-substrate enokuthi ifakwe i-hydrolyzed ngama-enzymes athile, okukhokelela ekukhululweni kwemveliso enemibala okanye ye-fluorescent.
Le khompawundi isetyenziswa ngokuqhelekileyo kwiimvavanyo zokubona ubukho kunye nomsebenzi wee-enzyme ezifana ne-beta-galactosidase kunye ne-beta-glucuronidase.Ezi enzymes ziqhekeza amaqela e-acetyl kunye ne-glucosaminide ukusuka kwi-substrate, ekhokelela ekubunjweni kwechromophore eluhlaza okwesibhakabhaka okanye eluhlaza.
Isakhiwo esiyingqayizivele se-5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide ivumela ukufumanisa lula kunye nokulinganisa umsebenzi we-enzyme.Ukusetyenziswa kwayo kwiindlela ezahlukeneyo zokulinga, kubandakanywa i-histochemistry, i-immunohistochemistry, kunye ne-cell-based assays, ibe negalelo ekuqondeni ngcono imisebenzi ye-enzyme.