I-5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS:4264-82-8

I-5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-Gluc) sisixhobo esisetyenziswa kakhulu ekufumaneni umsebenzi we-beta-glucuronidase (GUS). I-GUS yi-enzyme efumaneka kwizinto eziphilayo ezahlukeneyo, kuquka iibhaktheriya, izityalo, kunye nezilwanyana ezanyisayo. I-X-Gluc isetyenziswa rhoqo kuvavanyo lwe-GUS reporter kunye nophando lwebhayoloji yemolekyuli.

Eyona ndlela iphambili yokusebenzisa i-X-Gluc kukusebenzisa iindlela zokufaka idayi ye-histochemical, apho inokuyibona khona indlela esebenza ngayo i-enzyme ye-GUS. Le substrate ingena kwiseli kwaye inyibilikiswa yi-GUS, nto leyo ebangela ukuba kubekho imveliso eluhlaza okwesibhakabhaka okanye enganyibilikiyo. Le dayi eluhlaza okwesibhakabhaka ivumela abaphandi ukuba bachonge kwaye baqonde umsebenzi we-GUS kwiiseli, izicubu, kunye nezinto eziphilayo ezipheleleyo.

I-X-Gluc ingasetyenziswa nakwiimvavanyo zobungakanani ukulinganisa umsebenzi we-enzyme ye-GUS. Ubukhali bombala oluhlaza okwesibhakabhaka okanye ubungakanani bemveliso eyenziweyo bunokunxulunyaniswa nenqanaba lokubonakaliswa kwe-GUS okanye umsebenzi wayo we-enzyme.

Ukongeza, i-X-Gluc isetyenziswe kuphando lwezakhi zofuzo zezityalo ukufunda ukubonakaliswa kwezakhi zofuzo, umsebenzi wokukhuthaza, kunye nokuguqulwa kwezityalo. Ikwasetyenziswa kwiinkqubo zeebhaktheriya zokudibanisa nokufumanisa iiproteni ze-GUS fusion.