L-Leucine p-nitroanilide hydrochloride CAS:16010-98-3

I-L-Leucine p-nitroanilide hydrochloride isetyenziswa kakhulu njenge-substrate kuphando lwe-biochemical kunye novavanyo lwe-enzyme ukulinganisa umsebenzi wee-enzymes ezahlukeneyo, ngakumbi ii-proteases kunye nee-peptidases. Xa ezi enzymes ziqhekeza i-substrate, ikhupha i-p-nitroaniline, enokufunyanwa nge-spectrophotometric.

Izinga lokutshintsha kombala lihambelana ngqo nomsebenzi we-enzyme, nto leyo evumela ukuba kulinganiswe umsebenzi we-enzyme kwiisampuli zebhayoloji. Oku kwenza i-L-Leucine p-nitroanilide hydrochloride ibe sisixhobo esiluncedo sokufunda i-enzyme kinetics, ukuvavanya i-enzyme inhibitors okanye i-activators, kunye nokuvavanya imiphumo yeemeko zovavanyo okanye ukungenelela kumsebenzi we-enzyme.

Uvakalelo lwe-L-Leucine p-nitroanilide hydrochloride kwiimvavanyo ezahlukeneyo ze-enzyme lunokwahluka, lufuna ukulungiswa kwiinkqubo ezithile. Ihlala isetyenziswa kumanqanaba aqala kwi-0.5 mM ukuya kwi-5 mM, kuxhomekeke kwi-enzyme ethile kunye neemeko zovavanyo.