I-N-(1-Naphthyl)-3-aminopropanesulfonic Acid Ityuwa yeSodium I-CAS:104484-71-1

Isalathisi se-pH: Le khompawundi ibonisa utshintsho lombala oluxhomekeke kwi-pH, nto leyo eyenza ukuba ibe sisalathisi se-pH esiluncedo kwiimvavanyo ezahlukeneyo kunye neemvavanyo. Ingasetyenziswa ukujonga utshintsho lwe-pH kwiisampuli zebhayoloji, iimpendulo zeekhemikhali, kunye nezinye iinkqubo.

I-Fluorescent Probe: Ityuwa yesodium ye-N-(1-Naphthyl)-3-aminopropanesulfonic acid ineempawu ze-fluorescence, nto leyo eyenza ukuba ifaneleke kwizifundo ezisekelwe kwi-fluorescence kunye nokusetyenziswa kweelebheli. Ingafakwa kwiindlela zokuhlalutya zokubona nokulandelela iimolekyuli zebhayoloji okanye izakhiwo zeseli.

Uvavanyo lwe-Enzyme: Le khompawundi idla ngokusetyenziswa njenge-substrate okanye i-inhibitor kwii-enzyme assays, ingakumbi ezo zibandakanya amaqela e-sulfonate okanye umsebenzi we-enzyme ochaphazeleka lutshintsho lwe-pH. Inokunceda abaphandi bafunde i-enzyme kinetics, iindlela zokuthintela, kunye nokusebenzisana kwe-enzyme-substrate.

I-Buffer Component: I-N-(1-Naphthyl)-3-aminopropanesulfonic acid Ityuwa yesodium ingasetyenziswa njengenxalenye yeenkqubo ezithile ze-buffer, ingakumbi ezo zifuna iqela le-sulfonic acid okanye uluhlu oluthile lwe-pH. Inegalelo ekugcineni iimeko ze-pH ezifanelekileyo kwiisetingi zovavanyo.

Ukwenziwa kweeKhemikhali: Ngenxa yesakhiwo sayo esikhethekileyo seekhemikhali, ityuwa yesodium ye-N-(1-Naphthyl)-3-aminopropanesulfonic acid ingasebenza njengebhloko yokwakha okanye izinto zokuqala zokwenziwa kweemolekyuli ezahlukeneyo ze-organic okanye iikhompawundi zamayeza.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Izifundo zoBume beProteni: I-ANS inomdla omkhulu kwiindawo ezingathathi hlangothi kwiiproteni. Isetyenziswa kakhulu ekufundeni ukugoba kweproteni, ukuzinza, kunye notshintsho lokwakheka kwayo. Ukukhanya kwe-ANS kuyaphucuka xa ibopha kwiindawo ezingabonakaliyo kwiindawo ezingathathi hlangothi okanye iiproteni ezigobileyo, nto leyo eyenza ukuba ibe sisixhobo esibalulekileyo sokujonga ulwakhiwo lweproteni kunye nokuguquguquka kwayo.

Ukusebenzisana kweProtein-Ligand: I-ANS ingasetyenziselwa ukuphanda ukusebenzisana kweprotein-ligand, njengokubopha iimolekyuli ezincinci okanye amayeza kwiiproteni. Ukwanda okanye ukuncipha kwe-ANS fluorescence xa kukho i-ligand kunokubonelela ngolwazi malunga nokubopha kunye nendawo yokubopha indawo.

Izifundo zeMembrane: Ngenxa yokuba ayinamanzi, i-ANS isetyenziswa rhoqo ukufunda iiproteni zemembrane kunye nokusebenzisana kwe-lipid-protein. Ingaphanda iipropati ze-lipid bilayer, imisele i-topology yeprotein yemembrane, kwaye ijonge utshintsho kwi-membrane fluidity.

Uvavanyo lweProtein Denaturation: I-ANS fluorescence ingasetyenziselwa ukuvavanya i-protein denaturation okanye i-openings, njengakwiinkqubo ezibangelwa bubushushu okanye ze-chemical denaturation. Ukwanda kwe-ANS fluorescence kudla ngokubonwa njengoko iindawo ezingathathi cala emanzini zibonakala.

Ukuchongwa koQhagamshelo lweeProteni: I-ANS yaziwa ngokubophelela kwiiproteni ezihlanganisiweyo, ezifana nee-amyloid fibrils okanye ii-inclusion bodies. Ukwanda kobushushu be-ANS xa kukho iiproteni ezihlanganisiweyo kunokusetyenziswa ukujonga ukwakheka kunye nokuqhubela phambili kwezi zakhiwo.

Izifundo zokubopha ngeeNucleic Acids: I-ANS inokunxibelelana neenucleic acids, ingakumbi i-DNA enemicu emibini, kwaye ibonise ukukhanya. Isetyenziselwe ukufunda ukusebenzisana kwe-DNA-protein kunye notshintsho kwimo ye-DNA.

Ukufakwa kweelebheli zeFluorescent: I-ANS ingadityaniswa okanye idityaniswe kunye neemolekyuli ezinomdla ukwenza iiprobe zefluorescent kunye neelebheli. Oku kwenza kube lula ukubona nokulandelela iimolekyuli ezithile okanye izakhiwo zeseli.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C13H14NNaO3S
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	104484-71-1
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.