I-3-HYDROXY-4-(5-NITROPYRIDYLAZO)PROPYLANI CAS:143205-66-7

Yiprobe ye-fluorescent equlethe iqela le-nitrobenzoxadiazole (NBD), elibonisa iimpawu ezinamandla ze-fluorescence. Iqela le-aldehyde livumela ukulebhelwa kunye nokufunyanwa kwee-biomolecules ezifana neeproteni, ii-nucleic acids, kunye nee-lipids.

I-NBD-aldehyde idla ngokusetyenziswa njengedayi esabelayo kwiimvavanyo zokufumanisa nokulinganisa ii-protein okanye ii-peptide conjugation reactions, ukuguqulwa kweeproteni, kunye nokusebenzisana kweeproteni neeproteni. Ingabhala amaqela e-amino akhululekileyo kwiiproteni okanye ii-peptides, yenze ii-covalent bonds ezizinzileyo. Oku kuyenza ibe luncedo kwizicelo ezifana ne-immunocytochemistry, i-immunohistochemistry, kunye ne-western blotting.

I-fluorescence ye-NBD-aldehyde inokuvuselelwa kukukhanya kwe-ultraviolet okanye okwesibhakabhaka, kwaye ikhupha i-fluorescence eluhlaza. Le mpawu ivumela ukubonakala kunye nokulinganiswa kwee-biomolecules ezibhalwe ngamagama kusetyenziswa iindlela ze-fluorescence microscopy okanye i-spectroscopy.