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I-3-Morpholino-2-hydroxypropanesulfonic acid ityuwa yesodium CAS: 79803-73-9

I-3-Morpholino-2-hydroxypropanesulfonic acid ityuwa yesodium, eyaziwa ngokuba yityuwa yesodium ye-MES, yikhompawundi yemichiza esetyenziswa ngokuqhelekileyo njenge-buffering kuphando lwebhayoloji kunye ne-biochemical.

I-MES sisithinteli se-zwitterionic esisebenza njengomlawuli we-pH, egcina i-pH izinzile kwiinkqubo ezahlukeneyo zovavanyo.Inyibilika kakhulu emanzini kwaye inexabiso le-pKa elimalunga ne-6.15, nto leyo iyenza ilungele ukugqunyelelwa kuluhlu lwe-pH lwe-5.5 ukuya kwi-7.1.

Ityuwa yesodium ye-MES isetyenziswa rhoqo kubuchule bebhayoloji yemolekyuli njenge-DNA kunye ne-RNA yodwa, uvavanyo lwe-enzyme, kunye nokucocwa kweprotheyini.Ikwasetyenziswa kwimidiya yenkcubeko yeseli ukugcina indawo ezinzileyo ye-pH yokukhula kweeseli kunye nokwanda.

Enye into ephawulekayo ye-MES kukuzinza kwayo phantsi kweemeko ze-physiological kunye nokuchasana nokutshintsha kweqondo lokushisa.Oku kuyenza ilungele ukusetyenziswa kwimifuniselo apho kulindeleke ukuguquguquka kobushushu.

Abaphandi bahlala bekhetha ityuwa yesodium ye-MES njengesithinteli ngenxa yokuphazamiseka okuncinci kokusabela kwe-enzymatic kunye nomthamo ophezulu we-buffer ngaphakathi koluhlu lwe-pH olufanelekileyo.


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Isicelo kunye nesiphumo

Ukulawulwa kwe-pH: Ityuwa ye-sodium ye-MES isebenza njengomlawuli we-pH, inceda ukugcina indawo ezinzileyo ye-pH kwiinkqubo zokulinga.Isebenza ngokukodwa kuluhlu lwe-pH lwe-5.5 ukuya kwi-7.1.

Umthamo wokuBuffering: I-MES inomthamo ophezulu wokuthintela ngaphakathi kowona luhlu lwe-pH.Ichasa utshintsho kwi-pH nangona iivolumu ezincinci ze-asidi okanye isiseko zongezwa, zivumela ulawulo oluchanekileyo kwiimeko zovavanyo.

Uvavanyo lwe-Enzyme: I-MES iqhele ukusetyenziswa njengesithinteli kwiimvavanyo ze-enzyme ngenxa yokuphazamiseka okuncinci kokusabela kwe-enzymatic.Inceda ukugcina umsebenzi ofanelekileyo we-enzymatic ngokubonelela nge-pH ezinzileyo.

Ukucocwa kweeprotheyini: I-MES buffer isoloko isetyenziswa kwiinkqubo zokucocwa kweeprotheyini.Inceda ukugcina uzinzo kunye nomsebenzi weeprotheni ngexesha lamanyathelo ahlukeneyo okucoca, njenge-ion-exchange chromatography okanye i-gel filtration.

I-DNA kunye ne-RNA Isolation: I-MES isetyenziswe kwiinkqubo zokuzihlukanisa ze-DNA kunye ne-RNA, apho inceda ukugcina ukuzinza kwe-nucleic acids kunye ne-buffers ngokuchasene neenguqu ze-pH ezinokuchaphazela ingqibelelo yazo.

I-Cell Culture: ityuwa ye-sodium ye-MES isetyenziswa kwimidiya yenkcubeko yeseli ukugcina indawo ezinzileyo ye-pH ekhuthaza ukukhula kweeseli kunye nokwanda.Ibonelela ngesisombululo esithintelweyo esinceda ukugcina iimeko ezifanelekileyo zovavanyo lwenkcubeko yeseli.

Ukuzinza kunye nokuhambelana: I-MES iyaziwa ngokuzinza kwayo phantsi kweemeko ze-physiological kunye nokuchasana nokutshintsha kweqondo lokushisa.Ihlala isebenza kwiimeko ezahlukeneyo zovavanyo, iyenza ibe lukhetho olufanelekileyo kubaphandi.

Ukupakishwa kweMveliso:

6892-68-8-3

Iinkcukacha ezongezelelweyo:

Ukuqamba I-C7H16NNaO5S
Isivavanyi 99%
Imbonakalo Umgubo omhlophe
Inombolo yeCAS. 79803-73-9
Ukupakisha Incinci kwaye ininzi
Beka ubomi kwishelufa iminyaka eyi-2
Ugcino Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso ISO.

 


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