Ityuwa yesodium ye-3-Morpholino-2-hydroxypropanesulfonic acid CAS:79803-73-9

Ityuwa yesodium ye-3-Morpholino-2-hydroxypropanesulfonic acid, ekwaziwa ngokuba yi-MES sodium salt, yikhemikhali esetyenziswa kakhulu njenge-arhente yokukhusela kuphando lwebhayoloji kunye ne-biochemical.

I-MES yi-zwitterionic buffer esebenza njengomlawuli we-pH, igcina i-pH izinzile kwiinkqubo ezahlukeneyo zovavanyo. Inyibilika kakhulu emanzini kwaye inexabiso le-pKa elimalunga ne-6.15, nto leyo eyenza ifaneleke ukuhluzwa kuluhlu lwe-pH oluphakathi kwe-5.5 ukuya kwi-7.1.

Ityuwa yesodiyam ye-MES isetyenziswa rhoqo kwiindlela zebhayoloji yeemolekyuli ezifana nokwahlulwa kwe-DNA kunye ne-RNA, uvavanyo lwee-enzyme, kunye nokucocwa kweproteni. Ikwasetyenziswa kwimidiya yenkcubeko yeseli ukugcina imeko-bume ye-pH ezinzileyo yokukhula nokwanda kweseli.

Uphawu oluphawulekayo lwe-MES kukuzinza kwayo phantsi kweemeko zomzimba kunye nokuxhathisa utshintsho kumaqondo obushushu. Oku kuyenza ifaneleke ukusetyenziswa kwiimvavanyo apho kulindeleke ukuba kubekho utshintsho kumaqondo obushushu.

Abaphandi badla ngokuthanda ityuwa yesodium ye-MES njenge-buffer ngenxa yokuphazamiseka kwayo okuncinci kwiimpendulo ze-enzyme kunye nomthamo ophezulu we-buffer ngaphakathi koluhlu lwayo olufanelekileyo lwe-pH.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Ulawulo lwe-pH: Ityuwa yesodiyam ye-MES isebenza njengomlawuli we-pH, inceda ukugcina imeko-bume ye-pH ezinzileyo kwiinkqubo zovavanyo. Isebenza ngakumbi kuluhlu lwe-pH oluqala kwi-5.5 ukuya kwi-7.1.

Umthamo Wokubhafa: I-MES inomthamo ophezulu wokubhafa ngaphakathi koluhlu lwayo olufanelekileyo lwe-pH. Iyamelana notshintsho kwi-pH nokuba kongezwe imiqulu emincinci ye-asidi okanye isiseko, nto leyo evumela ulawulo oluchanekileyo kwiimeko zovavanyo.

Uvavanyo lwe-Enzyme: I-MES isetyenziswa kakhulu njenge-buffer kwiimvavanyo ze-enzyme ngenxa yokuphazamiseka kwayo okuncinci kwiimpendulo ze-enzyme. Inceda ukugcina umsebenzi we-enzyme ofanelekileyo ngokubonelela ngendawo ye-pH ezinzileyo.

Ukucocwa kweProtein: I-MES buffer isetyenziswa rhoqo kwiinkqubo zokucocwa kweprotein. Inceda ukugcina uzinzo kunye nomsebenzi weeprotein ngexesha lamanyathelo ahlukeneyo okucocwa, njenge-ion-exchange chromatography okanye i-gel filtration.

Ukuhlukaniswa kwe-DNA kunye ne-RNA: I-MES isetyenziswa kwiinkqubo zokwahlulwa kwe-DNA kunye ne-RNA, apho inceda ukugcina uzinzo lwee-nucleic acids kunye nee-buffers ngokuchasene notshintsho lwe-pH olunokuchaphazela ukuthembeka kwazo.

Ukukhula kweeSeli: Ityuwa yesodiyam ye-MES isetyenziswa kwimidiya yenkcubeko yeeseli ukugcina imeko-bume ye-pH ezinzileyo ekhuthaza ukukhula nokwanda kweeseli. Ibonelela ngesisombululo esihlanganisiweyo esinceda ukugcina iimeko ezifanelekileyo zovavanyo lwenkcubeko yeeseli.

Uzinzo kunye nokuhambelana: I-MES yaziwa ngokuzinza kwayo phantsi kweemeko zomzimba kunye nokumelana notshintsho lobushushu. Ihlala isebenza kwiimeko ezahlukeneyo zovavanyo, nto leyo eyenza ukuba ibe lukhetho olufanelekileyo kubaphandi.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C7H16NNaO5S
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	79803-73-9
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.