I-3-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE CAS:3150-25-2

Ukufunyanwa komsebenzi we-beta-galactosidase: I-ONPG idla ngokusetyenziswa ukufumanisa ubukho kunye nomsebenzi we-beta-galactosidase kwiisampuli ezahlukeneyo zebhayoloji, ezifana neenkcubeko zebhaktheriya okanye ii-cell lysates. Ukuveliswa kwe-o-nitrophenol, enombala otyheli, kunokulinganiswa ngokulula kusetyenziswa i-spectrophotometer.

Izifundo zokubonakaliswa kwezakhi zofuzo: I-ONPG isetyenziswa kakhulu kuphando lwebhayoloji yemolekyuli ukufunda ukubonakaliswa kwezakhi zofuzo. Ngokudibanisa umkhuthazi wezakhi zofuzo ezinomdla kunye nezakhi zofuzo ezifaka i-beta-galactosidase, abaphandi banokulinganisa umsebenzi wale mkhuthazi ngokongeza i-ONPG kunye nokulinganisa imveliso ye-o-nitrophenol ephumayo. Le ndlela, eyaziwa njenge-beta-galactosidase reporter assay, inika ulwazi malunga nomsebenzi wokubhalwa kwezakhi zofuzo.

Ukuchonga iintsholongwane: Ezinye iintsholongwane zivelisa i-beta-galactosidase, ngelixa ezinye zingenzi njalo. I-ONPG ingasetyenziswa kunye nezinye iimvavanyo zebhayoloji ukuchonga iintlobo zentsholongwane ngokusekelwe kubuchule bazo bokunyibilikisa i-ONPG. Le ndlela isetyenziswa kakhulu kwiilabhoratri zokuxilongwa kwezonyango kunye ne-microbiology.

Ukuhlolwa kwee-enzyme inhibitors okanye ii-activators: I-ONPG ingasetyenziselwa ukuvavanya ii-compounds ezilawula umsebenzi we-beta-galactosidase. Ngokulinganisa umsebenzi we-enzyme phambi kwee-compounds ezahlukeneyo, abaphandi banokuchonga ii-inhibitors okanye ii-activators ezinokubakho ezinokuphandwa ngakumbi malunga namandla azo okunyanga.