I-3,3′-Diaminobenzidine CAS:91-95-2

I-Immunohistochemistry: Ukufaka idayi ye-DAB kusetyenziswa kakhulu kwi-IHC ukufumanisa nokufumanisa iiproteni okanye ii-antigen ezithile kwiindawo zezicubu. Le ndlela inceda abaphandi kunye neengcali zezifo ukuba bafunde imo yeeseli, bachonge izinto zeseli, bahlole iipatheni zokubonakaliswa kweproteni, kwaye baphande utshintsho olunxulumene nesifo. Ukufaka idayi ye-DAB kungasetyenziswa kwiindawo ezifana nophando lomhlaza, i-neuroscience, i-pathology, kunye ne-biology yophuhliso.

I-Immunocytochemistry: Ukupenda i-DAB kukwasetyenziswa kwi-ICC ukuze kubonwe iimolekyuli ekujoliswe kuzo kwiiseli ezikhuliswe kakuhle. Le ndlela ifana ne-IHC, evumela abaphandi ukuba bahlole ukubonakaliswa kweproteni, indawo, kunye ne-cellular dynamics kwi-vitro.

Ukudityaniswa kwe-in situ: Ukudityaniswa kwe-DAB kungasetyenziswa ekufumaneni ulandelelwano oluthile lwe-DNA okanye lwe-RNA kusetyenziswa iindlela zokudityaniswa kwe-in situ. Ngokudibanisa ukudityaniswa kwe-DAB kunye neeprobes ezijolise kulandelelwano lwe-nucleic acid oluhambelanayo, abaphandi banokubona indawo kunye nokubonakaliswa kwezakhi zofuzo ngaphakathi kweeseli okanye kwizicubu.

I-electron microscopy: I-DAB staining ingasetyenziswa kwi-electron microscopy ukulebhelisha izakhiwo ezithile okanye iimolekyuli ezinomdla. Xa zidityaniswe ne-heavy metal post-staining, i-DAB staining inokuphucula umahluko kunye nokubonakala kwezixhobo zeseli kwizifundo ze-ultrastructural.