3,3′,5,5′-Tetramethylbenzidine CAS:207738-08-7 Umfanekiso Obonakalayo

I-3,3′,5,5′-Tetramethylbenzidine CAS:207738-08-7

I-3,3′,5,5′-Tetramethylbenzidine, ekwaziwa ngokuba yi-TMB, yikhemikhali esetyenziswa kakhulu njenge-chromogenic substrate kwi-enzyme-linked immunosorbent assays (ELISA) nakwezinye ii-biochemical assays. Ihlala isetyenziselwa ukufumanisa nokulinganisa ubukho bee-enzymes ezifana ne-horseradish peroxidase (HRP) kwiisampuli ezahlukeneyo zebhayoloji. I-TMB itshintsha umbala ukusuka ekubeni nombala ukuya kubhlowu xa kukho ezi enzymes. Emva koko, impendulo ingamiswa ngokongeza i-asidi eguqula umbala oluhlaza okwesibhakabhaka ube ngumbala wokugqibela otyheli. Ubunzulu bombala otyheli buhambelana nobungakanani be-enzyme ekhoyo, okuvumela ukulinganiswa.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

I-ELISA: I-TMB isetyenziswa rhoqo kwiimvavanyo ezisekelwe kwi-ELISA ukufumanisa nokulinganisa ubukho beeproteni, ii-antibodies, okanye ii-antigens. Ubungakanani bombala buhambelana ngokuthe ngqo noxinzelelo lwemolekyuli ekujoliswe kuyo.

Uvavanyo lomsebenzi we-enzyme: I-TMB ingasetyenziselwa ukulinganisa umsebenzi wee-enzyme ezifana ne-HRP. Ngokulinganisa izinga lokutshintsha kombala, abaphandi banokumisela umsebenzi okanye uxinzelelo lwe-enzyme kwisampuli.

Ukufaka umbala kwibhayoloji: I-TMB ingasetyenziswa kwi-immunohistochemistry kunye ne-immunocytochemistry ukuze kubonwe izicubu ezithile okanye iinxalenye zeseli. Xa i-TMB isabela kwi-enzyme HRP, ivelisa i-precipitate emdaka okanye eluhlaza okwesibhakabhaka kwindawo yemolekyuli ekujoliswe kuyo, okuvumela ukubonakala kwayo phantsi kwe-microscope.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C16H23ClN2O
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	207738-08-7
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.