I-4-Amino-3-hydrazino-1,2,4-triazol-5-thiol CAS:28836-03-5

Isiphumo se-3,3'-Diaminobenzidine (DAB) kukuvelisa umbala omdaka xa isabela kwiienzymes ze-peroxidase. Olu tshintsho lombala luvumela ukubonakala kwee-antigens ezijoliswe kuzo okanye ii-biomolecules ezinomdla kwi-immunohistochemistry (IHC) kunye nee-immunocytochemistry (ICC) staining assays. I-DAB idla ngokusetyenziswa kuphando lwe-biomedical kunye nezicelo zokuxilonga ukufumanisa nokufumanisa ii-antigens okanye iiproteni ezithile ngaphakathi kweesampuli zebhayoloji. Ngokongeza i-DAB njengesiseko kunye nokusabela okulandelayo ngee-enzymes ze-peroxidase, kwenziwa i-brown precipitate kwindawo yee-antigens ezijoliswe kuzo. Ukusetyenziswa kwe-DAB staining kuluncedo kakhulu ekufundeni izifo ezahlukeneyo kunye neemeko. Ivumela abaphandi kunye neengcali zezifo ukuba babone kwaye bahlalutye ukusasazwa, ubuninzi, kunye nendawo yee-biomolecules ezithile kwiithishu okanye kwiiseli. Olu lwazi lunceda ekuqondeni iindlela zesifo, ukufunda iinkqubo zeeseli, kunye nokuchonga iinjongo zokuxilongwa okanye zonyango ezinokubakho. Ngaphezu koko, ukudaya kwe-DAB kungasetyenziswa kwiindlela ezahlukeneyo ze-molecular ukuphanda ukusebenzisana kweproteni neproteni, ukuchonga iipatheni zokubonakaliswa kweproteni, ukumisela ubukho beempawu ezithile, kunye nokuvavanya ukusebenza kakuhle kokungenelela konyango. Ngenxa yokudaya kwayo okuthembekileyo nokuqinileyo, kunye nokuhambelana kwayo neenkqubo ezahlukeneyo zokufumanisa kunye neendlela ze-microscopy, i-DAB ibe yindawo esetyenziswa kakhulu kwi-chromogenic assays kwi-immunohistochemistry kunye ne-immunocytochemistry assays.