I-4-Nitrophenyl-alpha-D-glucopyranoside CAS:3767-28-0

I-substrate yovavanyo lwe-enzyme: I-4-Nitrophenyl-alpha-D-glucopyranoside isetyenziswa kakhulu njenge-substrate kwi-enzyme assays ukulinganisa umsebenzi we-glycosidases kunye nezinye ii-enzymes ezibandakanyeka kwi-carbohydrate metabolism. Ezi enzymes zinokuhlukanisa unxibelelwano phakathi kwe-glucose kunye neqela le-4-nitrophenyl, zikhuphe imveliso etyheli ebizwa ngokuba yi-4-nitrophenol. Ubungakanani be-4-nitrophenol eveliswayo buhambelana ngqo nomsebenzi we-enzyme, okuvumela ukulinganiswa kobungakanani bomsebenzi we-enzyme.

Ukufumanisa umsebenzi wee-enzyme: Ngokusebenzisa i-4-Nitrophenyl-alpha-D-glucopyranoside njenge-substrate, umsebenzi wee-enzyme ezithile unokulinganiswa. Umzekelo, ii-enzyme ze-glycosidase ezifana ne-beta-glucosidase, i-alpha-glucosidase, okanye i-beta-galactosidase zinokuqhekeza i-compound, zikhuphe i-4-nitrophenol, enokubonwa nge-photometric. Le ndlela isetyenziswa kakhulu kwiindlela ezahlukeneyo zonyango, amayeza, kunye ne-biotechnology.

Ukuhlolwa kwe-High-throughput: Ngenxa yokuhambelana kwayo neenkqubo zokufumanisa ezenzekelayo, i-4-Nitrophenyl-alpha-D-glucopyranoside isetyenziswa rhoqo kwiimvavanyo zokuhlola ze-high-throughput. Le khompawundi ivumela ukuchazwa ngokukhawuleza nangokufanelekileyo komsebenzi we-enzyme, nto leyo eyenza ukuba ibe luncedo ekufumaneni amayeza, ubunjineli be-enzyme, kunye nezifundo zokuthintela i-enzyme.

Ukusetyenziswa kokuxilonga: I-4-Nitrophenyl-alpha-D-glucopyranoside ingasetyenziswa kwizicelo zokuxilonga ukufumanisa ubukho okanye umsebenzi wee-enzyme ezithile ezinxulumene nezifo ezahlukeneyo. Umzekelo, umsebenzi wee-enzyme ezithile ungasetyenziswa njengophawu lokuxilonga izifo ezithile, kwaye i-4-Nitrophenyl-alpha-D-glucopyranoside ingasetyenziswa njengesiseko sokulinganisa umsebenzi wezi enzymes kwiisampuli zeklinikhi.