I-4-Nitrophenyl-beta-D-glucopyranoside CAS: 2492-87-7

Uvavanyo lomsebenzi we-Enzyme: I-hydrolysis ye-4-Nitrophenyl-beta-D-glucopyranoside ngama-enzymes afana ne-β-glucosidase ingasetyenziselwa ukulinganisa umsebenzi we-enzyme.Ukukhutshwa kwe-4-nitrophenol kunokulinganiswa usebenzisa i-spectrophotometry, evumela ukuzimisela kwe-enzyme kinetics kunye nezifundo zokuvimbela.

Ukuchazwa kweGene kunye novavanyo lweentatheli: I-4-Nitrophenyl-beta-D-glucopyranoside inokudibaniswa neethegi ezithile zemolekyuli ezidityaniswe kwiiprotheni ezidityanisiweyo.Umsebenzi we-enzyme weprotheyini yokudibanisa unokuthi ulinganiswe ngokulula ngokulinganisa ukukhutshwa kwe-4-nitrophenol, ukubonelela ngolwazi kwi-gene expression kunye ne-protein-protein interactions.

Ukufunyanwa kweziyobisi kunye nokuhlolwa kwe-high-throughput: I-hydrolysis ye-4-Nitrophenyl-beta-D-glucopyranoside ingasetyenziswa njenge-surrogate umsebenzi wokuvavanya ukufunyanwa kweziyobisi.Ivumela ukujongwa ngokukhawuleza kwamathala eencwadi amakhulu ekhompawundi ukuchonga izinto ezinokubakho ii-enzyme inhibitors okanye ii-activators.

Uvavanyo lokuxilonga: Ezinye izifo zinxulunyaniswa notshintsho kwimisebenzi ethile ye-enzyme.Ngokusebenzisa i-hydrolysis ye-4-Nitrophenyl-beta-D-glucopyranoside ngama-enzyme athile njengovavanyo lokuxilonga, ezi zifo zinokubonwa okanye zibekwe esweni.Oku kubandakanya iimeko ezinxulumene nokuphazamiseka kokugcinwa kwe-lysosomal kunye nezifo ze-hepatobiliary.