I-4-Nitrophenyl-beta-D-glucopyranoside CAS:2492-87-7

Uvavanyo lomsebenzi we-enzyme: I-hydrolysis ye-4-Nitrophenyl-beta-D-glucopyranoside yi-enzymes ezifana ne-β-glucosidase ingasetyenziselwa ukulinganisa umsebenzi we-enzyme. Ukukhululwa kwe-4-nitrophenol kunokulinganiswa kusetyenziswa i-spectrophotometry, okuvumela ukuqinisekiswa kwe-enzyme kinetics kunye nezifundo zokuthintela.

Uvavanyo lwe-gene expression kunye ne-reporter: I-4-Nitrophenyl-beta-D-glucopyranoside inokudityaniswa neethegi ezithile ze-molecular ezifakwe kwiiproteni ze-fusion. Umsebenzi we-enzyme weproteni ye-fusion unokulinganiswa ngokulula ngokulinganisa ukukhululwa kwe-4-nitrophenol, ukubonelela ngengqiqo malunga nokubonakaliswa kwe-gene kunye nokusebenzisana kweproteni neproteni.

Ukufunyanwa kwamayeza kunye nokuhlolwa kwe-high-throughput: I-hydrolysis ye-4-Nitrophenyl-beta-D-glucopyranoside ingasetyenziswa njengovavanyo lomsebenzi ongaphantsi kokufunyanwa kwamayeza. Ivumela ukuhlolwa ngokukhawuleza kweelayibrari ezinkulu ze-compound ukuchonga ii-enzyme inhibitors okanye ii-activators ezinokubakho.

Uvavanyo lokuxilonga: Ezinye izifo zinxulunyaniswa notshintsho kwimisebenzi ethile ye-enzyme. Ngokusebenzisa i-hydrolysis ye-4-Nitrophenyl-beta-D-glucopyranoside yi-enzymes ezithile njengovavanyo lokuxilonga, ezi zifo zinokufunyanwa okanye zijongwe. Oku kuquka iimeko ezinxulumene neengxaki zokugcina i-lysosomal kunye nezifo zesibindi.