I-5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide ityuwa yesodium CAS:129541-41-9

I-5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide ityuwa ye-sodium yi-chemical compound esetyenziswa ngokuqhelekileyo kuphando lwebhubhoratri kunye nokuxilongwa.Ihlala ibizwa ngokuba yi-X-Gluc kwaye isetyenziswa ngokubanzi njenge-substrate yokufumanisa umsebenzi we-enzyme ye-beta-glucuronidase.

Xa i-beta-glucuronidase ikhona, iqhekeza ibhondi ye-glucuronide kwi-X-Gluc, okubangela ukukhululwa kwedayi eluhlaza ebizwa ngokuba yi-5-bromo-4-chloro-3-indolyl.Oku kusabela ngokuqhelekileyo kusetyenziselwa ukubona okanye ukubona i-spectrophotometrically ukubonakaliswa kwe-enzyme ye-beta-glucuronidase kwiiseli okanye kwizicubu.

Ifom yetyuwa ye-sodium ye-X-Gluc iphucula ukunyibilika kwayo kwizisombululo ezinamanzi, iququzelele ukusetyenziswa kwayo kwiimvavanyo zebhubhoratri.I-X-Gluc isetyenziswa ikakhulu kuphando lwebhayoloji yemolekyuli ukufunda ukuchazwa kofuzo, umsebenzi wokukhuthaza, kunye novavanyo lofuzo lwentatheli.Ingasetyenziselwa ukufumanisa ubukho bezinto eziphilayo ezivelisa i-beta-glucuronidase, ezifana neebhaktheriya ezithile, kwizifundo ze-microbiological.

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Iinkcukacha zeMveliso

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Isicelo kunye nesiphumo

Ukufunyanwa kwe-GUS: I-X-Gluc ixutywe yi-GUS enzyme kwi-blue insoluble compound eyaziwa ngokuba yi-5-bromo-4-chloro-3-indole (X-Ind).Le mpendulo ivumela ukubonwa kunye nokulinganisa umsebenzi we-GUS kwiiseli kunye nezicubu.

Izifundo ze-Gene expression: I-X-Gluc isetyenziswa njengemolekyuli yengxelo kwizifundo zokuchazwa kofuzo.Ngokuxuba i-GUS gene kumgqugquzeli womdla, abaphandi banokumisela umsebenzi kunye nepateni yokubonisa indawo-yexeshana yomgqugquzeli ngokufumanisa umsebenzi we-GUS usebenzisa i-X-Gluc.

Uhlalutyo lwezityalo ze-Transgenic: Inkqubo ye-GUS reporter gene isetyenziswa ngokubanzi kwibhayoloji yemolekyuli yezityalo.I-X-Gluc staining ivumela abaphandi ukuba babone kwaye bafunde iipatheni zokubonisa i-transgene kwizityalo.Oku kunceda ekuqondeni ukulawulwa kofuzo, intetho ethe ngqo kwithishu, kunye nebhayoloji yophuhliso kwizityalo.

Ubunjineli be-Genetic: I-X-Gluc isetyenziswa njengophawu olukhethiweyo kwimifuniselo yobunjineli bemfuza.Ngokudibanisa i-GUS gene kwi-gene yangaphandle enomdla, i-X-Gluc staining ingasetyenziselwa ukuchonga ukuguqulwa okuyimpumelelo kunye nokudibanisa kweentlobo ezifunwayo kwi-organism.

Uphando lweMicrobiology: I-X-Gluc ingasetyenziselwa ukufumanisa nokuchonga ibhaktheriya evelisa i-GUS.I-enzyme ye-GUS ifumaneka kwiintlobo ezininzi zebhaktiriya ezahlukeneyo, kwaye ukungcoliswa nge-X-Gluc kuvumela ukubonwa kunye nokuchongwa kwe-GUS-positive ibhaktheriya kwizifundo ze-microbiological.

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Iinkcukacha ezongezelelweyo:

Ukuqamba	C14H14BrClNNaO7
Isivavanyi	99%
Imbonakalo	Umgubo omhlophe
Inombolo yeCAS.	129541-41-9
Ukupakisha	Incinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Ugcino	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	ISO.