Ityuwa ye-ammonium ye-8-Anilino-1-naphthalenesulfonic acid CAS:28836-03-5

I-8-Anilino-1-naphthalenesulfonic acid Ityuwa ye-ammonium yikhemikhali esetyenziswa kakhulu njengedayi ye-fluorescent. Inesakhiwo esenziwe liqela le-aniline eliqhotyoshelwe kwimolekyuli ye-naphthalenesulfonic acid enetyuwa ye-ammonium. Le khemikhali iyanyibilika emanzini kwaye ibonakalisa ukufunxwa okunamandla kunye nokukhupha ukukhanya okubonakalayo.

Ngenxa yeempawu zayo ze-fluorescent, ityuwa ye-ammonium ye-8-Anilino-1-naphthalenesulfonic acid idla ngokusetyenziswa njengeprobe okanye isalathisi kwizifundo ezahlukeneyo ze-biochemical kunye ne-biophysical. Ingasetyenziselwa ukubona utshintsho kwi-pH, utshintsho lwe-conformation kwiiproteni, ukusebenzisana kweproteni-ligand, kunye nokuba khona kwee-ion ezithile.

Xa ivuyiswa bubude bokukhanya obufanelekileyo, le khompawundi ikhupha ukukhanya okuluhlaza okwesibhakabhaka okuqaqambileyo, okuvumela ukuba kubonwe kwaye kulinganiswe lula. Iimpawu zayo zokukhanya ziyenza ibe sisixhobo esibalulekileyo kwiindawo zophando ezifana nebhayoloji yemolekyuli, i-biochemistry, kunye nebhayoloji yeseli.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Isalathisi se-pH: Le compound itshintsha incopho yayo yokukhupha i-fluorescence ngokuxhomekeke kwi-pH yesisombululo ekuso. Le mpawu iyenza ibe luncedo njengesalathisi se-pH kwizicelo ezahlukeneyo, ezinje ngokujonga utshintsho lwe-pH kwiisampuli zebhayoloji okanye ukufunda iimpendulo ze-enzyme.

Uhlalutyo lokwakheka kweproteni: Ityuwa ye-8-Anilino-1-naphthalenesulfonic acid ammonium inokubopha kwiiproteni kwaye itshintshe i-fluorescence intensity okanye ii-spectral properties ngokuxhomekeke kwimo yeproteni. Oku kuyenza ibe sisixhobo esiluncedo sokufunda ukugoba kweproteni, ukuguquka kwendalo, kunye notshintsho kwimo.

Ukusebenzisana kweprotein-ligand: Le compound ingasetyenziselwa ukufunda ukusebenzisana kweprotein-ligand. Ngokubophelela kwiprotein kwaye kutshintshe i-fluorescence xa ibopha i-ligand, ivumela abaphandi ukuba baphande nge-binding affinity, kinetics, kunye nendlela yokusebenza kwe-ligands ezahlukeneyo.

Ukufunyanwa kwee-ion: I-8-Anilino-1-naphthalenesulfonic acid ityuwa ye-ammonium isetyenzisiwe ukufumanisa nokulinganisa ii-ion ezahlukeneyo zesinyithi, ezifana ne-calcium, i-magnesium, kunye ne-zinc. Iimpawu ze-fluorescence zekhompawundi zinokuguqulwa bubukho bezi ions, nto leyo enika indlela ebukhali nekhethayo yokufunyanwa kwazo.

Ukubeka iliso kwindalo esingqongileyo: Le khompawundi isetyenziselwe iinjongo zokubeka iliso kwindalo esingqongileyo, njengokufumanisa ungcoliseko kunye nokujonga utshintsho kumgangatho wamanzi. Iimpawu zayo zokukhanya zivumela ukubonwa ngokukhawuleza nangokubonakalayo kongcoliseko kwiisampuli zamanzi.

Ikhemistri yohlalutyo: Ityuwa ye-8-Anilino-1-naphthalenesulfonic acid ammonium ikwasetyenziswa kwiindlela ezahlukeneyo zohlalutyo, ezifana ne-fluorescence spectroscopy kunye ne-high-performance liquid chromatography (HPLC), ukuze kulinganiswe kwaye kuchazwe iikhompawundi kwimixube enzima.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C16H16N2O3S
Uvavanyo	99%
Inkangeleko	Umgubo oluhlaza
Inombolo ye-CAS	28836-03-5
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.