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I-ABTS (2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) ityuwa yediammonium) CAS:30931-67-0

I-Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate), edla ngokubizwa ngokuba yi-ABTS, isetyenziswa ngokuqhelekileyo i-chromogenic substrate kwiimvavanyo ze-biochemical, ngokukodwa kwintsimi ye-enzymology.Yikhompawundi yokwenziwa esetyenziselwa ukulinganisa umsebenzi wee-enzymes ezahlukeneyo, kubandakanywa i-peroxidases kunye ne-oxidases.

I-ABTS ayinambala ngokwemo yayo ye-oxidized kodwa ijike ibe bluhlaza-luhlaza xa ifakwe i-enzayimu kwi-hydrogen peroxide okanye i-oxygen yemolekyuli.Olu tshintsho lombala lubangelwa ukubunjwa kwe-cation radical, ethatha ukukhanya kwi-spectrum ebonakalayo.

Ukusabela phakathi kwe-ABTS kunye ne-enzyme ivelisa imveliso enemibala enokulinganiswa nge-spectrophotometrically.Ubunzulu bombala buhambelana ngokuthe ngqo nomsebenzi we-enzymatic, okuvumela abaphandi ukuba bahlole ngokobungakanani be-enzyme kinetics, inhibition ye-enzyme, okanye i-enzyme-substrate interactions.

I-ABTS inoluhlu olubanzi lwezicelo kwiinkalo ezahlukeneyo, kubandakanya uxilongo lwezonyango, uphando lwamayeza, kunye nesayensi yokutya.Inobuntununtunu kwaye inika uluhlu olubanzi oluguquguqukayo, iyenza ibe lukhetho oludumileyo kwiimvavanyo ezininzi ze-biochemical.


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Isicelo kunye nesiphumo

Uvavanyo lwe-Enzymatic: I-ABTS isetyenziselwa ngokubanzi ukulinganisa umsebenzi wee-enzyme ezifana ne-peroxidases kunye ne-oxidases.Isebenza njenge-substrate yezi enzyme, kwaye umsebenzi wabo unokulinganiswa ngokulinganisa ubuninzi bemveliso enemibala eyenziwe.

I-Antioxidant Capacity Assays: I-ABTS idla ngokusetyenziswa kwii-antioxidant umthamo assays ukumisela amandla ezinto ukuba scavenge okanye inhibit radicals simahla.Ukwakhiwa kombala kubukho be-antioxidant kubonisa amandla ayo okutshabalalisa.

Uvavanyo lweeProtheyini: I-ABTS ingasetyenziselwa ukuvavanya umxholo weprotheyini epheleleyo kwiisampulu zebhayoloji.Ukusabela kwe-ABTS nge-protein-bound copper kubangela ukubunjwa kwemveliso enemibala enokuthi ilinganiswe.Le ndlela yaziwa ngokuba yi-bicinchoninic acid (BCA) assay.

Ukufunyaniswa kweziyobisi: I-ABTS isetyenziswa kwiimvavanyo zokuhlola i-high-throughput ukuvavanya imisebenzi ye-antioxidant yezinto ezinokuthi zibekho kwiikhompawundi zamachiza.Oku kuvumela abaphandi ukuba bachonge iikhompawundi ezineempembelelo zonyango ezinokubakho.

Umzi-mveliso wokutya neziselo: I-ABTS isetyenziswa kwishishini lokutya neziselo ukuze kuhlolwe umthamo we-antioxidant kwiimveliso ezahlukahlukeneyo zokutya, njengeziqhamo, imifuno kunye neziselo.Inceda ukuvavanya iinzuzo zempilo ezinokubakho kunye nokuzinza kwezi mveliso.

Ukubeka iliso kokusiNgqongileyo: I-ABTS ingaqeshwa ukuhlola umthamo opheleleyo we-antioxidant kwiisampulu zokusingqongileyo, inceda ekuvavanyeni amanqanaba ongcoliseko kunye nefuthe lawo kokusingqongileyo.

Ukupakishwa kweMveliso:

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Iinkcukacha ezongezelelweyo:

Ukuqamba I-C18H24N6O6S4
Isivavanyi 99%
Imbonakalo Umgubo oluhlaza
Inombolo yeCAS. 30931-67-0
Ukupakisha Incinci kwaye ininzi
Beka ubomi kwishelufa iminyaka eyi-2
Ugcino Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso ISO.

 


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