I-ONPG CAS:369-07-3 Ixabiso lomenzi

I-O-nitrophenyl-β-D-galactopyranoside (ONPG) yi-substrate yokwenziwa esetyenziswa kwiimvavanyo zebhayoloji kunye nezemolekyuli ukulinganisa umsebenzi we-enzyme i-β-galactosidase. Isetyenziswa rhoqo kwiimvavanyo ukufumanisa ukubonakaliswa kwezakhi zofuzo kwiinkqubo zebhaktiriya, ezifana ne-Escherichia coli. I-ONPG yi-compound engenambala eqhekeka yi-β-galactosidase, nto leyo ebangela ukukhululwa kwe-compound etyheli, i-o-nitrophenol. Umbala otyheli oveliswayo unokulinganiswa nge-spectrophotometric, ukubonelela ngomlinganiselo ongathanga ngqo womsebenzi we-enzyme. Uvavanyo olusebenzisa i-ONPG ludla ngokubizwa ngokuba yi-ONPG assay kwaye yindlela esetyenziswa kakhulu kuphando lwebhayoloji yeemolekyuli ukuvavanya amanqanaba okubonakaliswa kwezakhi zofuzo ezilawulwa yi-lac operon kwiiseli zebhaktiriya.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Isiphumo se-ONPG njengesiseko kufuneka sihlukaniswe yi-enzyme β-galactosidase, nto leyo ekhokelela ekukhutshweni kwemveliso etyheli, i-o-nitrophenol. Olu tshintsho lombala lunokulinganiswa nge-spectrophotometric, okuvumela ukulinganiswa komsebenzi we-β-galactosidase. Ukusetyenziswa kwe-ONPG ikakhulu kuvavanyo lokubonakaliswa kwezakhi zofuzo kwi-molecular biology kunye nophando lwe-microbiology. Isetyenziswa kakhulu ukulinganisa umsebenzi we-β-galactosidase njengentatheli yezifundo zokubonakaliswa kwezakhi zofuzo, ngakumbi kwiibhaktheriya ezifana ne-E. coli. I-lacZ gene, ebhala i-β-galactosidase, idla ngokusetyenziswa njengophawu lohlalutyo lokubonakaliswa kwezakhi zofuzo, njengoko ukubonakaliswa kwayo kunokubangelwa ziimeko ezithile okanye kulawulwe ngabakhuthazi abathile. Uvavanyo lwe-ONPG lubonelela ngendlela elula nethembekileyo yokuvavanya inqanaba lokubonakaliswa kwezakhi zofuzo ngokulinganisa umsebenzi we-β-galactosidase. Olu vavanyo lusetyenziswa kakhulu kwizicelo ezahlukeneyo, ezinje ngokufunda umsebenzi wokukhuthaza, ukulawulwa kwezakhi zofuzo, kunye nokusebenzisana kweproteni neproteni. Ukongeza, ingasetyenziselwa ukumisela i-enzyme kinetics kunye nokuvavanya imiphumo yokuguqulwa kwezakhi zofuzo okanye unyango kumsebenzi we-enzyme.

Isampuli yeMveliso

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C12H15NO8
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	369-07-3
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.