I-Nitrotetrazolium Blue Chloride CAS:298-83-9

I-Nitrotetrazolium Blue Chloride (NBT) sisalathisi se-redox esisetyenziswa kakhulu kwiimvavanyo zebhayoloji kunye nezebhayoloji. Yimpuphu etyheli ekhanyayo ejika ibe luhlaza okwesibhakabhaka xa incitshisiwe, nto leyo eyenza ukuba ibe luncedo ekufumaneni ubukho bee-enzyme ezithile kunye nomsebenzi we-metabolic.

I-NBT isabela kunye nabathwali bee-electron kunye nee-enzymes ezifana nee-dehydrogenases, ezibandakanyeka kwiinkqubo ezahlukeneyo zeseli. Xa i-NBT incitshiswa zezi enzymes, yenza i-blue formazan precipitate, evumela ukufunyanwa okubonakalayo okanye kwe-spectrophotometric.

Le reagent isetyenziswa rhoqo kwiimvavanyo ezifana novavanyo lokunciphisa i-NBT, apho isetyenziselwa ukuvavanya umsebenzi wokuqhuma kokuphefumla kweeseli zomzimba. Ingasetyenziselwa nokufunda imisebenzi ye-enzyme kunye neendlela ze-metabolic kuphando oluphathelele uxinzelelo lwe-oxidative, ukuphila kweseli, kunye nokwahluka kweseli.

I-NBT ifumene usetyenziso kwiinkalo ezahlukeneyo, kubandakanya i-microbiology, i-immunology, kunye ne-cell biology. Iyakwazi ukusetyenziswa ngeendlela ezahlukeneyo, izinzile, kwaye kulula ukuyisebenzisa, nto leyo eyenza ukuba ibe lukhetho oludumileyo kwiiprotokholi ezininzi zovavanyo.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Isiphumo esiphambili se-NBT kukwenziwa kwe-blue formazan precipitate xa incitshiswa zii-enzyme ezithile. Olu tshintsho lombala luvumela ukufunyanwa komsebenzi wee-enzyme ngokubonakalayo okanye nge-spectrophotometric.

I-NBT ineendlela ezahlukeneyo zokusetyenziswa kuphando kunye nokuxilongwa. Nazi ezinye zeendlela eziphambili zokusetyenziswa kwayo:

Uvavanyo lomsebenzi we-enzyme: I-NBT ingasetyenziselwa ukulinganisa umsebenzi wee-dehydrogenases, ezibandakanyeka kwiinkqubo ezifana nokuphefumla kweseli kunye ne-metabolism. Ngokujonga ukunciphisa kwe-NBT ibe yi-formazan, abaphandi banokuvavanya umsebenzi wezi enzymes.

Uvavanyo lomsebenzi weeseli zomzimba: I-NBT isetyenziswa rhoqo kuvavanyo lokunciphisa i-NBT ukuvavanya umsebenzi wokuqhuma kokuphefumla kweeseli zomzimba, ingakumbi ii-phagocytes. Olu vavanyo lulinganisa amandla ezi seli okuvelisa iintlobo zeoksijini ezisebenzayo, ezinokunciphisa i-NBT kwaye zenze i-blue precipitate.

Uphando lwe-microbiology: I-NBT isetyenziswa kwi-microbiology ukufunda i-metabolism ye-microbial kunye nokuvavanya umsebenzi wee-enzymes ezithile. Umzekelo, isetyenziselwe ukufumanisa i-bacterial nitrate reductases okanye iibhaktheriya ezenza i-formazan.

Izifundo zobomi beseli: Ukunciphisa i-NBT kuvumela abaphandi ukuba bavavanye umsebenzi we-metabolic kunye nobomi beseli. Ngokulinganisa ubunzulu bemveliso eluhlaza okwesibhakabhaka ye-formazan, kunokwenzeka ukumisela inani leeseli eziphilayo kwisampulu ethile.

Isampuli yeMveliso

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C40H30ClN10O6+
Uvavanyo	99%
Inkangeleko	Umgubo otyheli
Inombolo ye-CAS	298-83-9
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.