I-AMPPD CAS: 122341-56-4 Ixabiso lomenzi

I-AMPPD, mfutshane ngenxa I-3-(2′-spiroadamantane)-4-methoxy-4-(3″-phosphoryloxy)phenyl-1,2-dioxetane, sisixhobo se-chemiluminescent esisetyenziswa kakhulu kwizicelo zebhayoloji kunye ne-immunoassay. Isetyenziswa kakhulu kuphando lwebhayoloji yemolekyuli, ukuxilongwa, kunye nesayensi yobomi.

I-AMPPD yenzelwe ngokukodwa ukusetyenziswa kunye ne-alkaline phosphatase enzyme conjugates. Xa ivezwa kwi-alkaline phosphatase, i-AMPPD ifumana impendulo ekhawulezayo nesebenzayo ye-enzyme cleavage, ekhokelela ekukhutshweni kokukhanya.

Ukukhanya okukhutshiweyo okuvela kwi-AMPPD kubonwa kusetyenziswa iinkqubo zomfanekiso ezibuthathaka okanye ii-luminometers, okuvumela ukulinganiswa kunye nokubonwa kweemolekyuli okanye ii-analytes ekujoliswe kuzo. Le ndlela yokufumanisa esekelwe kwi-chemiluminescence inika uvakalelo oluphezulu, ingxolo esezantsi yangasemva, kunye noluhlu olubanzi lwe-dynamics.

Ngenxa yokuzinza kwayo kunye nesibonakaliso esihlala ixesha elide, i-AMPPD ifanelekile kwizicelo ezifana ne-Western blotting, i-ELISA (i-enzyme-linked immunosorbent assay), i-DNA hybridization, kunye ne-in situ hybridization. Ivumela abaphandi ukuba bafumane kwaye bahlalutye iiproteni, ii-nucleic acids, ii-antibodies, kunye nezinye ii-biomolecules.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

I-AMPPD idla ngokusetyenziswa kunye ne-enzyme alkaline phosphatase. Xa ivezwa kwi-alkaline phosphatase, i-AMPPD ifumana impendulo yokuqhekeka evelisa isignali yokukhanya. Olu kukhanya lukhutshwa lunokubonwa kwaye lulinganiswe kusetyenziswa izixhobo ezikhethekileyo, okuvumela ukubonakala kunye nokulinganisa ii-biomolecules ekujoliswe kuzo.

Ukusetyenziswa kwe-AMPPD kuxhaphake kakhulu kwiindlela ezahlukeneyo, kuquka i-Western blotting, i-ELISA (i-enzyme-linked immunosorbent assay), i-DNA kunye ne-RNA hybridization, kunye ne-in situ hybridization. Ezi ndlela zisetyenziselwa ukufunda iiproteni, ii-nucleic acids, ii-antibodies, kunye nezinye iimolekyuli zebhayoloji.

I-AMPPD inika iingenelo ezininzi, kubandakanya uvakalelo oluphezulu, ingxolo esezantsi engasemva, kunye noluhlu olubanzi lwe-dynamics. Isignali yayo ezinzileyo nehlala ixesha elide iyenza ifaneleke kuzo zombini uhlalutyo lomgangatho kunye nolwamanani.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C16H28N6O10P2
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	122341-56-4
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.