I-Avidin CAS:1405-69-2 Ixabiso lomenzi

Ukucocwa kweProteni: I-Avidin ingathintelwa kwinkxaso eqinileyo, efana ne-chromatography resin okanye ii-magnetic beads, kwaye isetyenziswe ukubamba iiproteni ze-biotinylated okanye ii-nucleic acids. Le ndlela yaziwa ngokuba yi-avidin affinity chromatography, kwaye ivumela ukucocwa kwee-molecule ezithile ezijoliswe kuzo ngendlela ethile.

IiProbes zeBiotinylated: I-Avidin idla ngokudityaniswa nee-enzymes, ii-fluorophores, okanye ezinye iilebheli kwaye isetyenziswa njenge-reagent yokubona kwiimvavanyo ezahlukeneyo. Iiprobes zeBiotinylated, ezifana nee-antibodies okanye ii-nucleic acid probes, zinokufunyanwa ngokubophelela kwiimolekyuli zokufumanisa ezidityaniswe ne-avidin. Le ndlela isetyenziswa rhoqo kwi-ELISA, kwi-immunohistochemistry, nakwi-nucleic acid hybridization assays.

Ukuhanjiswa Kwamayeza Ajoliswe Kuwo: I-Avidin ingasetyenziswa njengemolekyuli yokuthwala ukuhambisa amayeza okanye iiarhente zonyango ngokukodwa kwiiseli okanye kwiithishu eziveza i-biotin receptor. Ngokudibanisa i-biotin namayeza okanye ii-nanoparticles kunye nokuzidibanisa ne-avidin, iinkqubo zokuhanjiswa kwamayeza ajoliswe kuwo zinokwenziwa.

Iindlela zeBiology yeMolekyuli: Iinkqubo ezisekelwe kwi-Avidin zisetyenziswa kakhulu kuphando lwebhayoloji yemolekyuli. Umzekelo, i-streptavidin, ene-affinity efanayo ne-biotin njenge-avidin, isetyenziswa kwiindlela ezifana ne-DNA sequencing, i-PCR, kunye ne-in situ hybridization, apho idibene nee-probes okanye ii-primer ze-biotinylated.

Ukujonga nokuchonga: I-Avidin inokuphawulwa ngee-radioisotopes, iidayi ze-fluorescent, okanye ii-nanoparticles, nto leyo eyenza ukuba ibe sisixhobo esibalulekileyo kwiindlela zokujonga imifanekiso ezifana ne-positron emission tomography (PET), i-fluorescence microscopy, kunye ne-magnetic resonance imaging (MRI). Ukongeza, iinkqubo ezisekelwe kwi-avidin zisetyenziswa kwiimvavanyo zokuxilonga, ezifana ne-immunoassays, ukufumanisa ubukho be-biomarkers ezithile.