I-BCIP Na) 5-Bromo-4-chloro-3-indolyl phosphate disodium salt CAS:102185-33-1

Isiphumo:

I-BCIP yikhompawundi engenambala eguqulwa ibe yi-blu-purple precipitate xa i-alkaline phosphatase isebenza. Olu tshintsho lombala luvumela ukubonwa ngokubonakalayo kunye nokulinganiswa komsebenzi we-enzyme.

Izicelo:

Uvavanyo lwe-enzyme: I-BCIP isetyenziswa kwiindlela ezahlukeneyo zovavanyo lwe-biochemical ukulinganisa umsebenzi we-alkaline phosphatase. Oku kuquka ukubona umsebenzi we-phosphatase kwi-cell lysates, ii-enzymes ezicociweyo, okanye uvavanyo lwe-in vitro.

I-Immunohistochemistry: I-BCIP ingasetyenziselwa ukubona ii-antibodies ezibhalwe nge-alkaline phosphatase kwiindawo zezicubu okanye kumphezulu weseli. Le ndlela idla ngokusetyenziswa kwiiprotokholi zokufaka i-immunostaining.

Iindlela zokublotha: I-BCIP isetyenziswa kwi-Western blotting, Southern blotting, kunye ne-Northern blotting ukufumanisa iiproteni ezithile okanye ii-nucleic acids ezibhalwe nge-alkaline phosphatase-conjugated probes.

Ukudityaniswa kwe-in situ: I-BCIP ingasetyenziselwa ukubona iipatheni zokubonakaliswa kwezakhi zofuzo kwizicubu okanye kwiiseli ngeendlela zokudityaniswa kwe-in situ.

Uvavanyo lokuxilonga: I-BCIP isetyenziswa njengesiseko kwiikiti ezahlukeneyo zokuxilonga ukufumanisa i-alkaline phosphatase. Ezi kiti zisetyenziswa kwiilabhoratri zeklinikhi zokuxilonga nokubeka iliso kwisifo.