I-BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine ityuwa I-CAS:6578-06-9 Umfanekiso obonisiweyo

Ityuwa yeBCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine CAS:6578-06-9

Ityuwa ye-5-Bromo-4-chloro-3-indolyl phosphate p-toluidine yikhemikhali esetyenziswa kakhulu kwi-molecular biology kunye nophando lwe-biochemistry. Ihlala isetyenziswa kwi-enzyme-linked immunosorbent assays (ELISA) kunye neendlela zokubhala iilebhile zeproteni.

Le khompawundi yi-substrate ye-alkaline phosphatase, i-enzyme esetyenziswa kakhulu kwi-molecular biology ukuze kufunyanwe ubukho bee-molecules okanye iiproteni ezithile ngokusebenzisa i-colorimetric okanye i-chemiluminescent reactions.

Xa i-enzyme ye-alkaline phosphatase ikhona, iqhekeza amaqela e-phosphate kwityuwa ye-5-Bromo-4-chloro-3-indolyl phosphate p-toluidine, nto leyo ebangela ukwakheka kwedayi eluhlaza okwesibhakabhaka okanye emfusa. Olu tshintsho lombala lunokubonwa kwaye lulinganiswe lula, nto leyo evumela ukulinganiswa komsebenzi we-enzyme okanye ubukho beemolekyuli ekujoliswe kuzo.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

I-Western blotting: I-BCIP isetyenziselwa ukubona nokubona iiproteni ezidluliselwa kwiimembrane emva kwe-gel electrophoresis. Ii-antibodies zesibini ezidityaniswe ne-alkaline phosphatase zisetyenziselwa ukubopha kwii-antibodies eziphambili, kwaye emva koko i-incubation ene-BCIP iphumela ekuphuhlisweni kwe-blue precipitate kwindawo yee-protein bands.

I-Immunohistochemistry: I-BCIP isetyenziswa kwiindlela zokufaka idayi kwi-immunohistochemical ukufumanisa ubukho beeproteni ezithile kwizicubu. Ii-antibodies zesibini ezidityaniswe ne-alkaline phosphatase zisetyenziselwa ukubopha kwi-antibodies eziphambili, kwaye unyango lwe-BCIP luvelisa umbala oluhlaza okwesibhakabhaka xa kukho i-enzyme, nto leyo ebonisa indawo yeproteni kwisampuli yezicubu.

I-in situ hybridization: I-BCIP ikwasetyenziselwa ukubona ulandelelwano lwe-nucleic acid kwizicubu okanye kwiiseli ezizinzileyo. Ii-hybridization probes ziphawulwa nge-alkaline phosphatase, kwaye xa kongezwa i-BCIP, ubukho be-mRNA okanye i-DNA ekujoliswe kuyo buboniswa kuphuhliso lombala oluhlaza okwesibhakabhaka.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C8H6BrClNO4P.C7H9N
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	6578-06-9
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.