I-CHES Na CAS:103-47-9 Ixabiso lomenzi

I-2-(Cyclohexylamino)ethanesulfonic acid yikhemikhali equlethe ifomula yemolekyuli i-C10H21NO3S. Ikwaziwa ngesifinyezo sayo i-CHES. I-CHES sisiphumo se-sulfonic acid esiqulathe zombini iqela le-amino kunye neqela le-sulfonic acid kwisakhiwo sayo.

I-CHES isetyenziswa kakhulu njenge-arhente yokuthintela kuphando lwe-biochemical kunye ne-biological. Izinzile kwi-pH kwaye igcina imeko-bume ye-pH ehambelanayo, ngakumbi kwiindawo zelebhu ezibandakanya ii-enzyme reactions okanye izifundo zeproteni. I-CHES ine-pKa ye-9.3, nto leyo eyenza ukuba ibe yi-buffer esebenzayo malunga ne-pH ye-9.

Ulwakhiwo lwayo olukhethekileyo lweekhemikhali kunye neempawu zenza i-CHES ibe luncedo kwiindlela ezahlukeneyo zokusebenza, njengokulungiselela izisombululo ze-buffer ze-electrophoresis, uvavanyo lwe-enzyme, kunye ne-cell culture media. Ihlala ikhethwa kwiindlela ezifuna uluhlu lwe-pH oluyi-8.5 ukuya kwi-10.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Ukuhluza: I-CHES isetyenziswa kakhulu njengesixhobo sokunciphisa i-pH ukugcina i-pH izinzile kwiimvavanyo zebhayoloji kunye neempendulo ze-enzyme. Iluncedo ngakumbi kwizicelo ezifuna uluhlu lwe-pH oluyi-8.5 ukuya kwi-10.

I-Electrophoresis: I-CHES isetyenziswa rhoqo njenge-buffer kwiindlela ze-electrophoresis, ezifana ne-SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis), ukwahlula iiproteni ngokusekelwe kubunzima bazo beemolekyuli.

Uvavanyo lwe-enzyme: I-CHES isetyenziswa kwiimvavanyo ze-enzyme ukugcina i-pH efanelekileyo yomsebenzi we-enzyme. Inceda ukuqinisekisa ukuba ii-enzyme reactions zenzeka phantsi kweemeko ezilawulwayo nezithembekileyo.

I-Cell culture media: I-CHES ngamanye amaxesha ifakwa kwi-cell culture media kwiintlobo ezahlukeneyo zeeseli njengenxalenye elawula i-pH. Inceda ukugcina amanqanaba e-pH ephilayo, nto leyo ebaluleke kakhulu ekukhuleni nasekusebenzeni kweeseli ngokufanelekileyo.

Izifundo zeproteni: I-CHES idla ngokusetyenziswa kwiimvavanyo zokucocwa kweproteni kunye nokwenziwa kwekristale yeproteni. Iimpawu zayo zokubopha zinceda ukugcina uzinzo kunye nokuthembeka kweeproteni ngexesha lezi nkqubo.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C8H17NO3S
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	103-47-9
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.