I-Concanavalin A CAS:11028-71-0 Ixabiso lomenzi

I-Concanavalin A (Con A) yi-lectin, uhlobo lweproteni olunokubopha ngokukodwa kwiimolekyuli ezithile zeswekile. Ivela kwimbewu yesityalo se-jackbean (Canavalia ensiformis).

I-Con A inomdla omkhulu kwi-mannose kunye ne-glucose sugar residues kwaye inokubopha kwi-glycoproteins kunye ne-glycolipids eziqulethe ezi swekile. Yenza ii-complexes eziguqukayo kunye nezi molekyuli ngokusebenzisana kwe-carbohydrate-protein.

Ngenxa yokukwazi kwayo ukubopha iiswekile, iCon A isetyenziswa kakhulu kuphando lwebhayoloji kunye nokuxilongwa. Isetyenziswe kakhulu njengesixhobo sokufunda iiglycoproteins, iiswekile zomphezulu weseli, kunye nokusebenzisana okusekelwe kwicarbohydrates. Ingasetyenziselwa ukwahlula nokucoca iiglycoproteins nge-affinity chromatography, kunye nokufumanisa nokulinganisa iinxalenye ezithile zeswekile kumphezulu weseli okanye kwimixube enzima.

I-Con A ikwanazo neempawu zokulawula amasosha omzimba. Ingavuselela ukusebenza kunye nokwanda kweeseli ezithile zomzimba, ezifana nee-T lymphocytes, kwaye ifundwe ngezicelo zayo zonyango ezinokubakho kwi-immunotherapy kunye neendlela zokugonya.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Ukuxinana kweeSeli: Enye yeziphumo eziphawulekayo zeCon A kukukwazi kwayo ukubangela ukuxinana kweeseli. Le mpawu ibe luncedo ekufundeni ukunamathela kweeseli kunye nokusebenzisana komphezulu weseli.

Ukuhlukaniswa kweGlycoprotein: I-Con A ibopha ngokukodwa kwiiglycoproteins eziqulethe i-terminal mannose kunye neentsalela zeglucose. Le propati isetyenzisiwe ukwahlula nokucoca iiglycoproteins kwimixube enzima, efana nolwelo lwebhayoloji okanye ii-cell lysates, ngokusebenzisa i-Con A affinity chromatography.

Ukufunyanwa kweeGlycoproteins: I-Con A ingasetyenziselwa ukufumanisa nokulinganisa iiglycoproteins kwiisampuli. Ngokusebenzisa i-Con A edityaniswe neethegi ze-fluorescent okanye ze-enzymatic, kunokwenzeka ukubona okanye ukulinganisa ubungakanani beeglycoproteins ezikhoyo kwisampuli ngokusebenzisa i-fluorescence microscopy okanye iindlela ze-enzyme-linked immunosorbent assay (ELISA), ngokwahlukeneyo.

Ukulebhelisha Umphezulu Weseli: Ngenxa yokuba inomdla kwiiswekile zomphezulu weseli, iCon A ingasetyenziselwa ukulebhelisha iintlobo ezithile zeeseli okanye ukufunda iipateni zeglycosylation kumphezulu weseli. ICon A edityaniswe neefluorophores okanye ezinye iithegi ivumela ukubonakala kunye nohlalutyo lweeseli ngeendlela ezifana ne-flow cytometry okanye i-fluorescence microscopy.

Ukuguqulwa kwamasosha omzimba: I-Con A ineempawu zokuguqula amasosha omzimba kwaye inokuchaphazela ukusebenza kweeseli zomzimba. Ingavuselela ukusebenza kunye nokwanda kwee-T lymphocytes, nto leyo ekhokelela ekuvelisweni kwee-cytokines kunye nezinye iimpendulo zomzimba zomzimba. Le mpawu ihlolwe kunyango lwe-immunotherapy kunye nophuhliso lwe-vaccine, apho i-Con A isetyenziselwe ukuphucula iimpendulo zomzimba zomzimba okanye ukulungelelanisa ukunyamezela komzimba.

Isixhobo soPhando: I-Con A sisixhobo esisetyenziswa kakhulu kwiindawo ezahlukeneyo zophando lwebhayoloji. Sisetyenziselwe ukufunda ukusebenzisana kwe-carbohydrate-protein, ukunamathela kweeseli, iipatheni ze-glycosylation, kunye neempendulo zomzimba. Amandla ayo okunamathela kwiiswekile ezithile ayenza ibe luncedo ekufundeni nasekulawuleni ii-glycoproteins kunye nee-glycolipids.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C15H24
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	11028-71-0
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.