I-DAPI (4,6-Diamidino-2-phenylindole dihydrochloride) I-CAS:28718-90-3

I-DAPI (4,6-Diamidino-2-phenylindole dihydrochloride) yidayi ekhanyayo esetyenziswa kakhulu kuphando lwebhayoloji ukuze ibone i-DNA. Ibopha kwi-minor groove ye-DNA enemicu emibini kwaye ikhuphe i-blue fluorescence xa ikhanyiswa kukukhanya kwe-ultraviolet. I-DAPI idla ngokusetyenziswa kwi-fluorescence microscopy ukuze ifake i-nuclei yeseli, nto leyo eyenza ukuba ibe sisixhobo esibalulekileyo sokufunda ulwakhiwo kunye nokulungelelaniswa kwe-DNA ngaphakathi kweeseli. Ikwasetyenziswa kwi-flow cytometry ukuhlalutya umxholo we-DNA kwiiseli nganye nakwezinye iindlela zokubona i-DNA kunye nokulinganisa. Ubudlelwane obuphezulu be-DAPI kwi-DNA kunye neempawu zayo ezithile ze-fluorescent zenza ukuba ibe sisixhobo esiguquguqukayo nesisetyenziswa kakhulu kuphando lwe-molecular kunye ne-cell biology.