I-DDT CAS:3483-12-3 Ixabiso lomenzi

I-DL-Dithiothreitol, ekwaziwa ngokuba yi-DTT, yiarhente yokunciphisa esetyenziswa kakhulu kuphando lwebhayoloji ye-biochemical kunye ne-molecular. Yimolekyuli encinci eneqela le-thiol (eliqulathe i-sulfur) kwicala ngalinye.

I-DTT isetyenziswa rhoqo ukuphula ii-disulfide bonds kwiiproteni, nto leyo enceda ekuzivuleni okanye ekuziguquleni. Oku kunciphisa ii-disulfide bonds kubalulekile kwiinkqubo ezahlukeneyo zelebhu ezifana nokucocwa kweeproteni, i-gel electrophoresis, kunye nezifundo zesakhiwo seproteni. I-DTT ingasetyenziselwa ukukhusela amaqela e-thiol kunye nokuthintela i-oxidation ngexesha leenkqubo zovavanyo.

I-DTT idla ngokufakwa kwizisombululo zovavanyo ngobuninzi obuncinci, kwaye umsebenzi wayo uxhomekeke ekubeni ikhona ioksijini. Kubalulekile ukuyiphatha ngononophelo i-DTT kuba inovelwano emoyeni, kubushushu, nakumswakama, nto leyo enokunciphisa ukusebenza kwayo.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

Ukunciphisa iiBond zeDisulfide: I-DTT isetyenziselwa kakhulu ukuphula iibond ze-disulfide, ezizibond ze-covalent ezenziwe phakathi kwee-residues ezimbini ze-cysteine kwiiproteni. Ngokunciphisa ezi bond, i-DTT inceda ukuguquguqula iiproteni, ivumela ukufundwa kwesakhiwo kunye nomsebenzi wazo.

Ukugoqeka kweProtein: I-DTT inokunceda ekugoqekeni kweprotein ngokufanelekileyo ngokuthintela ukwakheka kwe-disulfide bond engalunganga. Inciphisa naziphi na ii-disulfide bonds ezingezizo ezendalo ezinokuthi zenziwe ngexesha lokugoqeka kweprotein, ivumela iprotein ukuba ithathe ukwakheka kwayo kwendalo.

Umsebenzi we-Enzyme: I-DTT ingenza ii-enzyme ezithile zisebenze ngokunciphisa naziphi na iibhondi ze-disulfide ezithintelayo ezikhoyo. Ukongeza, i-DTT inokuthintela i-oxidation yee-residues ezibalulekileyo ze-cysteine, ezinokuba yimfuneko kumsebenzi we-enzyme.

Ukuveliswa kwee-Antibody: I-DTT idla ngokufakwa ukunciphisa ii-disulfide bonds ngexesha lokuveliswa kwee-antibodies. Inceda ekuthinteleni ukwakheka kwee-disulfide bonds ezingalunganga, nto leyo enokuthintela ukubopha kwe-antigen ngokufanelekileyo.

Ukuzinzisa iiProteni: I-DTT ingasetyenziselwa ukuzinzisa iiproteni ngokuthintela i-oxidation okanye ukuhlangana kwazo. Inceda ukugcina imeko ephantsi yeeproteni ngexesha lokugcinwa kunye neenkqubo zovavanyo.

Iiarhente zokunciphisa kwi-Molecular Biology: I-DTT idla ngokusetyenziswa kwiindlela ezahlukeneyo ze-molecular biology ezifana nokulandelelana kwe-DNA, i-PCR, kunye nokuhlanjululwa kweproteni. Inokunceda ukugcina imeko ephantsi yezinto ezibalulekileyo, ukuqinisekisa iziphumo ezilungileyo zovavanyo.

Isampuli yeMveliso

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C4H10O2S2
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	3483-12-3
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.