I-Fluorescein Isothiocyante CAS:3326-32-7

Iimpawu zeFluorescence: I-FITC ine-quantum yield ephezulu kwaye ikhupha i-fluorescence eluhlaza xa ivuselelwa malunga ne-488 nm. Iimpawu zayo ze-fluorescence zenza ukuba ifaneleke ukubonwa kusetyenziswa iindlela ezahlukeneyo zokuthatha imifanekiso, ezifana ne-fluorescence microscopy, i-flow cytometry, kunye ne-fluorescence spectroscopy.

Ukubopha nge-covalent: I-FITC iqulethe iqela le-isothiocyanate elivumela ukuba libophe nge-covalent kumaqela e-amino akhoyo kwiiproteni, ii-antibodies, kunye nezinye ii-biomolecules. Oku kubopha nge-covalent kuqinisekisa ukulebhelwa okuzinzileyo nokungqalileyo kwee-molecules ekujoliswe kuzo, okuvumela ukuba zibonwe kwaye zibonakale.

I-Immunofluorescence: Ii-antibodies ezineleyibhile ye-FITC zisetyenziswa rhoqo kwiimvavanyo ze-immunofluorescence ukufumanisa ii-antigens okanye iiproteni ezithile kwiiseli kunye nezicubu. I-fluorescence eluhlaza ekhanyayo ekhutshwa yi-FITC ivumela abaphandi ukuba baqonde kwaye babone ukusasazwa kwee-molecules ekujoliswe kuzo ngaphakathi kweesampuli zebhayoloji.

I-Flow cytometry: Ii-antibodies ezibhalwe yi-FITC okanye ezinye ii-biomolecules ezihlanganiswe yi-fluorochrome zisetyenziswa kakhulu kwi-flow cytometry ukuhlalutya nokwahlula iiseli ngokusekelwe kwiimpawu zazo zomphezulu okanye izinto ezingaphakathi kweseli. I-FITC iyahambelana ne-488 nm laser esetyenziswa rhoqo kwizixhobo ze-flow cytometry.

Izifundo zokusebenzisana kweeproteni: I-FITC ingasetyenziswa kwizifundo zokusebenzisana kweeproteni ukuvavanya ukubopha kunye nokusebenzisana kweeproteni. Ngokubhala iiproteni ezithile nge-FITC, abaphandi banokuhlalutya ukusebenzisana kwazo besebenzisa iindlela ezifana nokudluliselwa kwamandla e-fluorescence resonance (FRET) okanye i-fluorescence polarization.

Ukulandelela iimolekyuli: I-FITC ingasetyenziselwa ukulandelela nokulandelela intshukumo yeemolekyuli eziphilayo kwiiseli okanye kwiithishu. Ngokubhala iimolekyuli ezinomdla kwi-FITC, abaphandi banokufunda indawo yazo, amandla azo, kunye neepatheni zokurhweba ngexesha langempela besebenzisa i-fluorescence microscopy.

Ukusetyenziswa kokuxilonga: Iiprobe okanye ii-antibodies ezineleyibhile ye-FITC zisetyenziswa ekuxilongeni kwiindlela ezahlukeneyo zokusebenza, kubandakanya ukufumanisa ii-antigens ezithile, ii-agents ezisulelayo, okanye ii-biomarkers kwiisampuli zesigulana. Ezi probe ze-fluorescent zibonelela ngokuchonga okunobunono nokuchanekileyo, okuvumela ukuxilongwa ngokukhawuleza nangokuchanekileyo.