I-FLUORESCEIN MONO-BETA-D- GALACTOPYRANOSIDE CAS:102286-67-9

I-Fluorescein mono-beta-D-galactopyranoside, eyaziwa ngokuba yi-FMG, yi-compound ekhanyayo esetyenziswa kakhulu njenge-substrate kwiimvavanyo ezahlukeneyo ze-biochemical kunye ne-cell biology. Ivela kwi-methyl-beta-D-galactopyranoside ngokuyidibanisa ne-molecule ye-fluorescein. I-FMG isetyenziswa kakhulu ekufundeni umsebenzi we-beta-galactosidase, i-enzyme evuselela i-hydrolysis ye-lactose kwi-galactose kunye ne-glucose. Ngokusebenzisa i-FMG njenge-substrate, abaphandi banokujonga umsebenzi we-enzyme we-beta-galactosidase ngokulinganisa ukukhutshwa kwe-fluorescence. I-hydrolysis ye-FMG yi-beta-galactosidase ikhokelela ekukhutshweni kwe-fluorescein, okubangela ukwanda kwesignali ye-fluorescent enokulinganiswa. Le compound ikwasetyenziselwa ukuphanda ukuqondwa kwe-carbohydrate kunye nokusebenzisana. I-FMG ingasetyenziselwa njenge-molecular probe ukufunda i-binding affinity ye-lectins (iiproteni ezibopha ngokukodwa kwi-carbohydrates) kwi-galactose-containing carbohydrates. Ukubopha kwe-FMG-lectin complexes kunokubonwa kwaye kulinganiswe ngokusekelwe kutshintsho ekukhutshweni kwe-fluorescence. Ngokubanzi, i-FMG sisixhobo esiguquguqukayo ekufundeni umsebenzi we-enzyme kunye nokuqondwa kwe-carbohydrate, enika indlela elula neyobuchule yokulinganisa i-fluorescence kunye nokuvavanya ezi nkqubo zebhayoloji.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

I-Fluorescein mono-beta-D-galactopyranoside (FMG) yimolekyuli esetyenziswa kakhulu kuphando lwebhayoloji njengesiseko sokufumanisa ubukho kunye nomsebenzi we-enzyme ye-beta-galactosidase. I-FMG sisiphumo se-lactose yeswekile kwaye idityaniswe nemolekyuli ye-fluorescein.

Isiphumo esiphambili se-FMG kukuba i-hydrolyzed ngokukodwa yi-beta-galactosidase, i-enzyme eqhekeza i-lactose ibe yi-galactose kunye ne-glucose. Le hydrolysis ye-enzyme ye-FMG ikhokelela ekukhutshweni kwe-fluorescein, ekhupha isignali enamandla ye-fluorescence.

Eyona ndlela iphambili yokusebenzisa i-FMG kukufumanisa nokulinganisa umsebenzi we-beta-galactosidase kwiisampuli ezahlukeneyo. Le enzyme ifumaneka kwizinto ezininzi eziphilayo, kuquka iibhaktheriya kunye neeseli zezilwanyana ezincancisayo, kwaye umsebenzi wayo unokubonisa iinkqubo ezahlukeneyo zeseli kunye neendlela zemetabolism.

Ngokusebenzisa i-FMG njenge-substrate, umsebenzi we-beta-galactosidase unokulinganiswa ngokujonga i-fluorescence ekhutshwa yi-fluorescein ekhululweyo. Olu vavanyo lunokwenziwa kwiindlela ezahlukeneyo zovavanyo, kuquka uvavanyo lwe-in vitro kunye nezifundo zomfanekiso weseli ephilayo.

Ngaphezu koko, i-FMG ingasetyenziswa njengesixhobo sokufunda ukusasazwa kunye nendawo ye-beta-galactosidase ngaphakathi kweeseli. Ngokusebenzisa iindlela ze-fluorescent microscopy, abaphandi banokubona i-fluorescence ekhutshwa yi-FMG xa i-hydrolysis ingasebenzi, nto leyo ebavumela ukuba balandelele umsebenzi wendawo kunye nowexesha we-beta-galactosidase.

Isampuli yeMveliso

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C26H22O10
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	102286-67-9
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.