I-Geneticin disulfate (i-G418 sulfate) I-CAS:108321-42-2

Kwiindawo zelebhu, i-geneticin disulfate (i-G418 sulfate) isetyenziswa kakhulu kwiimvavanyo zenkcubeko yeseli ukukhetha nokugcina imigca yeseli equlethe iivektha zokubonakalisa ezineejini zokumelana, ezifana ne-neomycin phosphotransferase. Ezi seli zinokuphila xa kukho i-geneticin disulfate ngenxa yokubonakaliswa kwejini yokumelana, okuvumela abaphandi ukuba bahlukanise kwaye bafunde ii-populations zeeseli eziguqulwe ngokwezakhi zofuzo. Abaphandi basebenzisa i-geneticin disulfate kwizicelo ezahlukeneyo, kubandakanya ukuveliswa komgca weseli ozinzileyo, iindlela zokuhlela izakhi zofuzo ezifana ne-CRISPR-Cas9, kunye nokuveliswa kweproteni ephinda-phindayo. Ngokufaka i-geneticin disulfate kwindawo yokukhulisa, ii-populations ezithile zeeseli zinokutyetyiswa okanye zohlulwe ngokusekelwe kubuchule bazo bokusinda ekukhethweni kwee-antibiotic. Ukuphucula ukuxinana kunye nobude bonyango lwe-geneticin disulfate kubalulekile ekufezekiseni uxinzelelo olukhethiweyo ngelixa kunciphisa i-cytotoxicity kunye neziphumo ezingajoliswanga kwiiseli ezingadluliselwanga. Ukubeka esweni ngononophelo impilo yeseli, iimpawu zokukhula, kunye neepateni zokubonakalisa izakhi zofuzo kunceda ukuqinisekisa iziphumo zovavanyo eziphumelelayo xa kusetyenziswa i-geneticin disulfate kwizifundo zenkcubeko yeseli. Abaphandi kufuneka balandele imigaqo-nkqubo esekwe yokusetyenziswa kwe-geneticin disulfate, kubandakanya iinkqubo ezifanelekileyo zokulinganisa, ukuphatha, kunye nokulahla, ukugcina imigangatho yokhuseleko lwelabhoratri kunye nokukhuthaza iziphumo zesayensi ezichanekileyo. Intsebenziswano phakathi koososayensi, abasebenzi belabhoratri, kunye nabasemagunyeni abalawulayo ixhasa ukusetyenziswa okufanelekileyo nokusebenzayo kwe-geneticin disulfate kuphando lwebhayoloji yemolekyuli, okuqhubela phambili ukuqonda kwethu iinkqubo zeseli kunye neendlela zokulawula i-genetic.