HEPBS CAS:161308-36-7 Ixabiso lomvelisi

N-(2-Hydroxyethyl)piperazine-N'-(4-butanesulfonic acid) (I-HEPBS) sisithinteli se-zwitterionic esiqhele ukusetyenziswa kuphando lwebhayoloji nakwimichiza.Isiphumo salo esiphambili kukunceda ukugcina i-pH ezinzileyo kwizisombululo, ngokukodwa ngaphakathi kwe-physiological pH range (7.2-7.4).

Esona sicelo siphambili seI-HEPBS ikwinkcubeko yeseli, apho isetyenziswa njengenxalenye yemidiya yenkcubeko ukugcina i-pH yesisombululo.Inceda ukubonelela ngendawo ezinzileyo yokukhula kweeseli kunye nokuthintela nakuphi na ukuguquguquka kwe-pH okunokuthi kube yingozi kwiiseli.

I-HEPBS Ikwaqashwa njenge-agent ephazamisayo kwizifundo ze-enzyme, njengoko inokuzinzisa i-pH ngexesha lokusabela kwe-enzymatic.Ngokuqhelekileyo isetyenziselwa ukuhlanjululwa kweeprotheyini kunye neemvavanyo ze-enzymatic ukwenzela ukwandisa umsebenzi kunye nokuzinza kwee-enzymes.

Ngaphezu koko,I-HEPBS isetyenziswa kwiindlela ezahlukeneyo ze-electrophoretic, ezifana ne-gel electrophoresis kunye ne-capillary electrophoresis, ukugcina i-pH efunwayo kunye nokuzinzisa iimolekyuli ezihlawulisiweyo ezihlulwe.

Ukongeza kwiipropathi zayo ze-buffer,I-HEPBS inokuphinda isebenze njenge-inhibitor ebuthathaka ye-metalloproteins ethile kunye ne-enzymes, eyenza ibe luncedo kwizicelo ezithile.