I-HEPES-Na CAS:75277-39-3 Ixabiso lomenzi

Ityuwa yesodium ye-HEPES, ekwaziwa ngokuba yi-N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid sodium salt, yi-zwitterionic buffer esetyenziswa kakhulu kuphando lwebhayoloji kunye ne-biochemical. Umsebenzi wayo ophambili kukugcina inqanaba le-pH elizinzileyo kwiinkqubo ezahlukeneyo zovavanyo. Ityuwa yesodium ye-HEPES iguquguqukayo kakhulu, izinzile, kwaye ayinatyhefu, okwenza ifaneleke kwiintlobo ngeentlobo zezicelo kubandakanya inkcubeko yeseli, i-enzyme kinetics, i-immunoassays, kunye novavanyo lwebhayoloji yemolekyuli. Ihambelana neesampuli ezininzi zebhayoloji kunye neenkqubo, ibonelela ngendawo elawulwayo yokulawula i-pH ngokuchanekileyo.

Oda okanye Cela iKoteshini

Iinkcukacha zeMveliso

Iithegi zeMveliso

Ukusetyenziswa kunye nesiphumo

I-arhente yokufunxa: Ityuwa yesodiyam ye-HEPES isetyenziswa kakhulu njenge-arhente yokufunxa kwizilingo zebhayoloji kunye nezebhayoloji. Inceda ukugcina uluhlu lwe-pH oluzinzileyo, ngakumbi kuluhlu lwe-physiological (pH 7.2-7.6). Amandla ayo okufunxa ayenza ibe luncedo ekugcineni iimeko ezifanelekileyo kwiimpendulo ezahlukeneyo ze-enzyme, inkcubeko yeseli, kunye neendlela zebhayoloji yemolekyuli.

Inkcubeko yeeseli: Ityuwa yesodiyam yeHEPES isetyenziswa kakhulu njenge-arhente yokukhusela kwiiseli. Ukukwazi ukugcina i-pH ezinzileyo kubalulekile ekukhuleni okufanelekileyo nasekuphileni kweeseli. I-HEPES idla ngokukhethwa ngaphezu kwezinye ii-arhente zokukhusela kuba ayibonisi tshintsho lubalulekileyo kwi-pH xa ivezwa kwi-CO2 esemoyeni.

Izifundo ze-enzyme: Ityuwa yesodiyam ye-HEPES iluncedo kakhulu kwizifundo ze-enzyme apho kufuneka indawo ye-pH ehlala ihleli nelawulwayo. Ithintela ukuguquguquka okukhulu kwi-pH ngexesha leempendulo ze-enzyme, iqinisekisa umsebenzi ofanelekileyo we-enzyme.

I-Electrophoresis: Ityuwa yesodium ye-HEPES isetyenziswa kakhulu njenge-arhente yokubopha kwiindlela ezahlukeneyo ze-electrophoretic ezifana ne-polyacrylamide gel electrophoresis (PAGE) kunye ne-agarose gel electrophoresis. Inceda ukugcina i-pH ye-buffer, ebaluleke kakhulu ekwahlukaniseni nasekuhlalutyeni ii-nucleic acids kunye neeproteni.

Uvavanyo lwe-biochemical: Ityuwa ye-HEPES sodium idla ngokusetyenziswa kwiindidi ezahlukeneyo zovavanyo lwe-biochemical, kubandakanya uvavanyo lwe-enzyme, uvavanyo lwe-protein quantification, kunye novavanyo lwe-spectrophotometric. Inceda ukugcina uluhlu lwe-pH olufunekayo ukuze kufunyanwe iziphumo ezichanekileyo neziphindaphindwayo.

Ukwenziwa kwamayeza: Ityuwa yesodiyam yeHEPES ikwasetyenziswa kwifomyula zamayeza njengearhente yokuthintela ukuzinzisa iifomyula zamayeza nokugcina uluhlu lwe-pH olufunekayo.

Ukupakisha imveliso:

Iinkcukacha ezongezelelweyo:

Ukwakhiwa	C8H19N2NaO4S
Uvavanyo	99%
Inkangeleko	Umgubo omhlophe
Inombolo ye-CAS	75277-39-3
Ukupakisha	Encinci kwaye ininzi
Beka ubomi kwishelufa	iminyaka eyi-2
Indawo yokugcina izinto	Gcina kwindawo epholileyo neyomileyo
Isiqinisekiso	I-ISO.