I-MOBS CAS: 115724-21-5 Ixabiso lomvelisi

Umenzeli wokuBhafa:I-MOBS isetyenziselwa ukugcina i-pH ezinzileyo kwisisombululo, ngokukodwa kwi-neutral ukuya kwi-alkaline encinci (pH 6.5-7.9).Ichasa utshintsho kwi-pH ebangelwa ukongezwa kwe-acids okanye iziseko, okwenza ukuba ifaneleke kwizicelo ezahlukeneyo apho kufuneka khona i-pH eqhubekayo.

Inkcubeko yeSeli:I-MOBS isetyenziswa rhoqo njenge arhente yesithinteli kumajelo eendaba enkcubeko yeeseli.Inceda ukugcina eyona pH ilungileyo yokukhula kweeseli kunye nokusebenza.

Iimvavanyo ze-Enzyme:I-MOBS isetyenziswe kwiimvavanyo ze-enzyme ukubonelela nge-pH ezinzileyo.Iqinisekisa ukuba umsebenzi we-enzyme awuchaphazeleki ngokuguquguquka kwe-pH, ukuvumela umlinganiselo ochanekileyo we-enzyme kinetics kunye nomsebenzi.

I-Electrophoresis:I-MOBS isetyenziswa ngokuqhelekileyo kwiindlela ze-electrophoresis, ezifana ne-agarose gel electrophoresis kunye ne-polyacrylamide gel electrophoresis (PAGE).Inceda ukugcina i-pH efunekayo kwi-buffer esebenzayo, ukuphucula isisombululo kunye nokwahlula i-DNA, i-RNA, okanye iiprotheni.

Ubuchule bebhayoloji yeMolekyuli:I-MOBS isetyenziswa kwiindlela ezahlukeneyo zebhayoloji yemolekyuli njenge-DNA kunye ne-RNA yodwa, i-PCR, kunye ne-RNA electrophoresis.Ibonelela ngeemeko ze-pH ezingaguqukiyo nezizinzileyo ezifunekayo kwezi nkqubo.

Ukucocwa kweprotheyini:I-MOBS inokusetyenziswa njengesithinteli kwiinkqubo zokucoca iiprotheyini, apho ukugcinwa koluhlu lwe-pH olufunekayo kubalulekile ekugcineni uzinzo kunye nomsebenzi weprotheyini.